摘要
目的分析血清miR-200c表达水平与上皮性卵巢癌(EOC)患者治疗后复发的关系。方法选取2019年6月至2020年6月昆明医科大学第三附属医院收治的160例EOC患者作为研究对象,随访2年,有104例复发。调查患者的基线资料,采用实时荧光定量聚合酶链反应(qRT-PCR)法检测患者血清miR-200c的表达水平,采用单因素分析患者治疗后复发与血清miR-200c的表达水平和基线资料的相关性,多因素Logistic回归分析影响患者复发的独立危险因素,绘制受试者工作特征(ROC)曲线分析miR-200c预测EOC患者治疗后复发的效能。结果单因素分析显示,雌激素受体、孕激素受体、淋巴结转移、血清糖类抗原125(CA125)和人附睾蛋白4(HE4)的水平与EOC患者治疗后复发呈正相关,血清miR-200c水平与EOC患者治疗后复发呈负相关(P<0.05);多因素Logistic回归分析显示,血清CA125高表达(OR>1,P<0.05)、miR-200c低表达(OR<1,P<0.05)是EOC患者术后复发的独立危险因素;ROC曲线结果显示,血清CA125、miR-200c水平单独及联合预测EOC患者治疗后复发的曲线下面积分别为0.915、0.954、0.993,血清miR-200c的预测效能优于CA125,联合预测效能优于单项指标。结论miR-200c是提示EOC细胞侵袭性的分子标志物,血清miR-200c表达水平的降低会增加EOC患者治疗后复发的风险,可作为潜在的癌症复发预测指标和治疗靶点。
Objective To analyze the relationship between serum miR-200c and recurrence after treatment in epithelial ovarian cancer(EOC)patients.Methods A total of 160 EOC patients admitted to the Third Affiliated Hospital of Kunming Medical University from June 2019 to June 2020 were selected as the study subjects.Among them 104 cases of recurrence were found during a 2-year follow-up.The serum miR-200c was measured by quantitative real time polymerase chain reaction(qRT-PCR),and its baseline data were investigated.Univariate analysis of the correlation between recurrence after EOC treatment and serum miR-200c and baseline data in patients;multivariate Logistic regression analysis of independent risk factors affecting patient recurrence;receiver operating characteristic(ROC)curve analysis predicts the efficacy of recurrence in EOC patients after treatment.Results Univariate analysis showed that the levels of estrogen receptor,progesterone receptor,lymph node metastasis,serum carbohydrate antigen 125(CA125)and human epididymis protein 4(HE4)were positively correlated with the recurrence of EOC patients after treatment,but the serum miR-200c was negatively correlated with the recurrence of EOC patients after treatment(P<0.05).The multivariate Logistic regression analysis showed that high serum CA125 expression(OR>1,P<0.05)and low miR-200c expression(OR<1,P<0.05)were independent risk factors for postoperative recurrence in EOC patients.The ROC curve results showed that the serum CA125 and miR-200c levels alone and combination predicted recurrence in EOC patients after treatment was 0.915,0.954,and 0.993.The predictive efficacy of serum miR-200c was superior to CA125,and the combined predictive efficacy was superior to single indicators.Conclusions MiR-200c indicates the aggressiveness of EOC cells.The decrease in serum miR-200c will increase the risk of recurrence in EOC patients after treatment,and can serve as a potential predictive indicator and treatment target for cancer recurrence.
作者
王丽雯
李静芳
李晓红
余鑫
WANG Liwen;LI Jingfang;LI Xiaohong;YU Xin(Department of Laboratory,the Third Affiliated Hospital of Kunming Medical University,Kunming 650118,Yunnan,China)
出处
《中国性科学》
2024年第3期58-61,共4页
Chinese Journal of Human Sexuality
基金
云南省教育厅科学研究基金项目(2019J1277)。
