摘要
目的:比较南京溧水2个黑莓品种'Hull'和'Chester'次生代谢产物和抗氧化活性差异。方法:采用UPLCQTOF-MS/MS全息表征'Hull'和'Chester'黑莓果实中的次生代谢产物,采用UV-Vis测定两者总酚、总花青素、总原花青素、总黄酮、总三萜酸含量,并对比其抗氧化活性。结果:2种黑莓中共鉴定出42个化合物,矢车菊素-3-O-芸香糖苷仅在'Hull'中检测到,矢车菊素-3-O-木糖苷仅在'Chester'中检测到,野鸦椿酸的2种异构体分别在2个品种各自检测到。定量结果显示,2个品种中总酚、总黄酮和总原花青素含量无显著差异,但'Chester'总花青素含量比'Hull'高20.8%,而'Hull'总三萜酸含量比'Chester'高55.3%。抗氧化能力对比发现,两者都具有强大的自由基清除能力,且'Hull'DPPH和ABTS自由基清除率和IC50值均优于'Chester'。结论:2个品种内在质量和活性均存在一定差异。这些差异是否影响黑莓深加工产品质量还有待深入研究。
Objective:To compare the secondary metabolites profiles and antioxidant activity between'Hull'and'Chester',two blackberry cultivars in Lishui district of Nanjing.Methods:An UPLC-QTOF-MS/MS based non-targeted plant metabolomics method was established to characterize the chemical profiles of'Hull'and'Chester'.The contents of total phenols,total flavonoids,total proanthocyanidins,total anthocyanin and total triterpenoid acids were quantified by UV-Vis.Then,the antioxidant activity between two cultivars was evaluated.Results:A total of 42 components were identified.Cyanidin-3-O-rutinoside was detected only in'Hull',and cyanidin-3-O-xyloside was only in'Chester'.Two euscaphic acid isomers were respectively detected in two blackberry cultivars.Moreover,the contents of total phenols,total flavonoids and total proanthocyanidins had no significant difference between two cultivars.The content of total anthocyanin in'Chester'was 20.8%higher than that in'Hull',while the content of total triterpenoid acids in'Hull'was 55.3%higher than that in'Chester'.The results of antioxidant activity showed that both had strong free radical scavenging ability,while the scavenging rates and IC50 values of'Hull'on DPPH and ABTS radical were better than'Chester'.Conclusion:Both the compositions contents of bioactive substances and antioxidant activity were different between'Hull'and'Chester'.Whether the quality difference between two blackberry cultivars affected the qualities and bioactivities of their processed products need further investigation.
作者
郝彩凤
周姗姗
王俊杰
徐金娣
毛茜
朱贺
郑学瑜
李松林
孔铭
Hao Caifeng;Zhou Shanshan;Wang Junjie;Xu Jindi;Mao Qian;Zhu He;Zhen Xueyu;Li Songlin;Kong Ming(Department of Pharmaceutical Analysis and Metabolomics,Affiliated Hospital of Integrated Traditional Chinese and Western Medicine,Nanjing University of Chinese Medicine,Nanjing 210028,China;Department of Pharmaceutical Analysis and Metabolomics,Jiangsu Province Academy of Traditional Chinese Medicine,Nanjing 210028,China;Jiangsu Taizhou People's Hospital,Taizhou 225300,China;Nanjing Meihao Biotechnology Co.,Ltd.,Nanjing 210000,China)
出处
《中国野生植物资源》
CSCD
2024年第3期9-19,共11页
Chinese Wild Plant Resources
基金
江苏省中医药科技发展计划(MS2022035)
南京中医药大学自然科学基金(XZR2020031)。
关键词
黑莓
次生代谢产物
抗氧化
定性
定量
Blackberry
Secondary metabolites
Antioxidant
Qualify
Quantify
