摘要
目的:探讨通管方对输卵管炎性不孕(SI)模型大鼠输卵管组织中Fas/FasL通路的影响。方法:采用经阴道内接种混合菌液的方法,建立输卵管炎性不孕大鼠模型。造模后,77只SD雌性大鼠随机分成空白组、假手术组、模型组、妇可靖胶囊组(0.29 g·kg^(-1)·d^(-1))、通管方低剂量组(7.515 g·kg^(-1)·d^(-1))、通管方中剂量组(15.03 g·kg^(-1)·d^(-1))、通管方高剂量组(30.06 g·kg^(-1)·d^(-1)),各组大鼠分别灌胃给药28 d后处死并取材。采用蛋白免疫印迹法(WB)检测SI模型大鼠输卵管组织中Fas、FasL蛋白及Caspase-1的表达水平,酶联免疫吸附法(ELISA)检测SI模型大鼠血清IL-4、IL-10、Caspase-1的表达变化。结果:与空白组比较,模型组血清中IL-4和IL-10的水平均明显降低,血清和输卵管组织中的Caspase-1水平明显升高(P<0.01);与模型组比较,通管方各剂量组和妇可靖胶囊组降低了血清和输卵管组织中的Caspase-1的表达(P<0.01),上调了血清中IL-4和IL-10水平、输卵管组织中Fas和FasL蛋白的表达(P<0.05,P<0.01)。其中,通管方高剂量组的作用均明显优于其他剂量组和妇可靖胶囊组(P<0.05,P<0.01)。结论:通管方可能通过调控Fas/FasL凋亡信号通路而消除SI模型大鼠输卵管炎症。
Objective:To explore the effect of Tongguan formula(TGF)on the Fas/FasL pathway in the fallopian tube tissue of rats with salpingitis induced infertility(SI).Methods:Rat model of tubal inflammatory infertility was established by injecting mixed bacterial solution through the vagina.After modeling,77 SD female rats were randomly divided into a blank group,a sham operation group,a model group,a Fu Ke Jing(FKJ)capsule group(0.29 g/kg·d^(-1)),a low dose group of TGF(7.515 g/kg·d^(-1)),a medium dose group of TGF(15.03 g/kg·d^(-1)),and a high dose group of TGF(30.06 g/kg·d^(-1)).After 28 days of gavage administration,the rats in each group were euthanized and samples were taken.Protein immunoblotting(WB)was used to detect the expression levels of Fas,FasL protein,and Caspase-1 in the fallopian tube tissue of SI model rats.Enzyme-linked immunosorbent assay(ELISA)was used to detect the expression changes of IL-4,IL-10,and Caspase-1 in the serum of SI model rats.Results:Compared with the blank group,the levels of IL-4 and IL-10 in the serum of the model group were significantly reduced,while the level of Caspase-1 in the serum and fallopian tube tissue was significantly increased(P<0.01).Compared with the model group,the expression of Caspase-1 in serum and fallopian tube tissue was reduced in each dose group of TGF and the FKJ group(P<0.01),and the levels of IL-4 and IL-10 in serum,and the expression of Fas and FasL proteins in fallopian tube tissue were upregulated(P<0.05,P<0.01).Among them,the high-dose group of TGF had significantly better effects than other traditional Chinese medicine control groups and the FKJ capsule group(P<0.05).Conclusion:Tongguan formula may eliminate salpingitis in SI model rats by regulating the Fas/FasL apoptotic signaling pathway.
作者
刘慧敏
王青
李盛男
徐佳
匡继林
LIU Huimin;WANG Qing;LI Shengnan;XU Jia;KUANG Jilin(Hunan University of Chinese Medicine,Changsha 410208,China;The Second Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410005,China)
出处
《中医药学报》
CAS
2024年第4期29-33,共5页
Acta Chinese Medicine and Pharmacology
基金
湖南省中医药科研计划重点课题(202012)。
