摘要
目的:探索种植体周围软组织与口腔黏膜组织的差异,揭示种植体周围微环境特征。方法:从GEO数据集下载基因芯片数据GSE43744,运用生物信息学工具对大鼠种植体周围组织和正常口腔黏膜组织的差异基因分析。结果:筛选获得1315个重要差异基因,其中797个基因表达上调,518个基因表达下调。基因富集分析显示,相较正常口腔黏膜组织,种植体周围软组织先天免疫活动、细胞活化、炎症反应以及对外界刺激和对细菌的刺激反应相关功能基因表达显著上调,细胞外基质组织、粘附、细胞外基质多糖,对机械刺激的反应,对有毒物质的反应等相关功能表达显著下调。T细胞相关的多个分子功能和生物途径高表达,可能在种植体周围微环境中发挥重要作用。构建了PPI网络并筛选了7个枢纽基因,包括FCER1G、TYROBP、PTPRC、ITGB2、AIF1、EMR1、RAC2,这些基因可能是种植体周围微环境研究的关键基因。结论:种植体周围软组织与正常口腔黏膜中基因表达差异显著,且具有独特的微环境特征,利用PPI网络筛选的枢纽基因,可能是未来种植体周围微环境研究的关键靶点。
Objective:To explore the differences of microenviroment between peri-implant tissue and oral mucosal tissue.Methods:The gene chip data GSE43744 was downloaded from the GEO database,bioinformatics tools were used to analyze the differentially expressed genes between the peri-implant tissue and normal oral mucosal tissue in rat.Results:1315 differentially expressed important genes,including 797 upregulated genes and 518 downregulated genes,were screened out.Gene enrichment analysis showed that compared with normal oral mucosal tissue,the gene expression of innate immune activity,cell activation,inflammatory response,and functional expression related to external and bacterial stimuli in peri-implant tissue were significantly upregulated,while that of extracellular matrix tissue,adhesion,extracellular matrix polysaccharides,response to mechanical stimuli and response to toxic substances was significantly downregulated.Meanwhile,multiple molecular functions and biological pathways related to T cells were highly expressed,which may play an important role in the peri-implant microenvironment.In addition,PPI network was constructed,and screened 7 core genes including FCER1G,TYROBP,PTPRC,ITGB2,AIF1,EMR1 and RAC2,which may be target genes for studying peri-implant microenvironment.Conclusion:There is a significant difference of microenvironment characteristics between peri-implant tissue and oral mucosa.The target genes screened using PPI network may be the key to future research on the peri-implant microenvironment.
作者
葛泽阳
陈鹏
李晨玉
王依柔
刘毅
王菁
陈骥
李德华
GE Zeyang;CHEN Peng;LI Chenyu;WANG Yirou;LIU Yi;WANG Jing;CHEN Ji;LI Dehua(State Key Laboratory of Oral&Maxillofacial Reconstruction and Regeneration,National Clinical Research Center for Oral Diseases,Shaanxi Engineering Research Center for Dental Materials and Advanced Manufa,Department of Oral Implantation,The Third Hospital Affiliated of Air Force Military Medical University,710032 Xi'an,China)
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2024年第2期187-192,共6页
Journal of Practical Stomatology
基金
国家自然科学基金青年科学项目(编号:82100969)
空军军医大学第三附属医院科技发展基金(编号:2020JZ081)。
关键词
种植体
微环境
差异基因
生物信息学分析
Dental implant
Microenvironment
Differential genes
Bioinformatics analysis
