摘要
目的探索改良型富血小板纤维蛋白(A-PRF)促进脂肪移植存活的最适混合比例。方法酶消化法分离脂肪干细胞并培养,传代至第三代,抽取健康患者静脉血一次性制备A-PRF,以1:6、1:8、1:10、1:12、1:14五种混合比例分别与脂肪干细胞混合。取6周龄SPF级雌性裸鼠60只,随机分为5组,每组12只,分别将A-PRF以体积比1:6、1:8、1:10、1:12、1:14五种混合比例分别与人脂肪颗粒混合后注射至5组裸鼠背部,分别记为1:6、1:8、1:10、1:12、1:14组。于移植后4、8、12周以断颈法处死裸鼠,比较脂肪标本体积及质量变化;HE染色观察脂肪标本的血管密度及分布;分析囊腔/空泡占比、炎症反应程度、间质化纤维程度;免疫组化测定CD31蛋白含量。结果第24小时,5组迁移变化均不明显,在第48小时及72小时1:12组的细胞迁移程度明显高于其他组,差异具有统计学意义(P<0.05)。在第4、8及12周,1:12组的脂肪质量组织体积及质量的保留率最高,差异具有统计学意义(P<0.05)。HE染色结果显示,1:12组的囊腔/空泡占比、间质化纤维程度均为最低并伴有大量新生毛细血管,5组在第4周时炎性反应程度均较严重,在第8及第12周的炎性反应程度均较轻且差异不明显。CD31染色结果显示,1:12组的CD31蛋白含量较其他组明显升高,差异具有统计学意义(P<0.05)。结论改良型富血小板纤维蛋白促进脂肪移植存活的最适混合比例是1:12。
Objective To explore the optimal mixing ratio of advanced platelet-rich fibrin to promote the survival of fat transplantation.Methods Adipose-derived stem cells were isolated by enzymatic digestion and cultured.A-PRF was prepared from venous blood of healthy patients.A-PRF was mixed with adipose-derived stem cells at five mixing ratios of 1:6,1:8,1:10,1:12,and 1:14 for wound healing assay.Sixty 6-week-old female immunodeficient mice were injected with A-PRF mixed with human fat granules at the volume ratio of 1:6,1:8,1:10,1:12,and 1:14,respectively.The mice were observed continuously for twelve weeks.The volume and mass of fat samples were compared,and the density and distribution of blood vessels were observed by HE staining.The ratio of cyst/vacuole,the degree of inflammatory reaction,and the degree of interstitial fibers were analyzed.Results At 48 hours,the cell migration rate of 1:6,1:8,1:10,1:12,1:14 group were(17.67±2.49)%,(21.60±0.79)%,(24.25±0.48)%,(67.98±2.24)%and(30.55±1.18)%,respectively.At 72 hours,the migration rates of 1:6,1:8,1:10,1:12,1:14 w(30.78±1.06)%,(35.95±1.29)%,(65.47±1.64)%,(83±3.72)%and(64.12±1.99)%,respectively.The degree of cell migration in the 1:12 mixture ratio group was significantly higher than that in the other concentration groups,and the difference was statistically significant(P<0.05).The retention rate of fat mass and volume in the 1:12 mixture ratio group was the highest in twelve weeks,and the difference was statistically significant(P<0.05).HE staining showed that the inflammatory reaction of the five experimental groups was more severe in the first four weeks,and the inflammatory reaction was less severe in the eight and twelve weeks,and the difference was not significant.The 1:12 mixed ratio group had the lowest ratio of cyst/vacuole,the lowest degree of interstitial fibers,and a large number of new capillaries.CD31 showed that the protein content of CD31 in the 1:12 mixture ratio group was significantly higher than that in the other groups,and the difference was statistically significant(P<0.01).Conclusion The optimal mixing ratio of advanced platelet-rich fibrin to promote the survival of fat transplantation is 1:12.
作者
刘翔宇
彭海涛
尹海林
田超
文银亭
贺红林
丁娜
袁怡君
李均
LIU Xiangyu;PENG Haitao;YIN Hailin;TIAN Chao;WEN Yinting;HE Honglin;DING Na;YUAN Yijun;LI Jun(Department of Burn and Plastic Surgery,Nanchong Central Hospital,The Second Clinical Medical College of North Sichuan Medical College,Nanchong 637000,Sichuan,China;Department of Obstetrics and Gynecology,Nanchong Central Hospital,The Second Clinical Medical College of North Sichuan Medical College,Nanchong 637000,Sichuan,China)
出处
《西部医学》
2024年第4期506-511,共6页
Medical Journal of West China
基金
南充市市校科技战略合作项目(20SXQT0301,20SXQT0320,22SXQT0344,22SXQT0134,22SXQT0121)
南充市科技局项目(22YYJCYJ0074)。
关键词
自体脂肪移植
改良型富血小板纤维蛋白
炎症反应
存活率
Autologous fat transplantation
Advanced platelet-rich fibrin
Inflammatory response
Survival rate
