摘要
目的 检测不同浓度的伊曲康唑对人血管瘤内皮细胞凋亡的影响,为伊曲康唑治疗血管瘤的机制研究提供依据。方法 体外培养的人血管瘤内皮细胞(HemECs)经不同浓度伊曲康唑干预不同时间后,倒置显微镜观察HemECs形态学变化;CCK-8法检测HemECs细胞抑制率;流式细胞术检测伊曲康唑诱导HemECs的凋亡率。结果 当伊曲康唑浓度≥30μg/mL时,作用效果逐渐显著,浓度与细胞凋亡呈正相关性,各浓度的抑制率和空白组相比差异均有统计学意义(P<0.05)。伊曲康唑干预时间与对HemECs细胞增殖的抑制和凋亡呈正相关。结论伊曲康唑能抑制HemECs增值有抑制作用,并促进其凋亡。浓度越高,干预时间越长对HemECs的细胞增殖的抑制作用和细胞凋亡的促进作用会越显著。浓度≥30μg/mL时,效果会逐渐显著,与细胞凋亡和抑制细胞增殖呈正相关性,24 h为较为适宜的干预时间。
Objective To detect the effects of different concentrations of itraconazole on apoptosis of human hemangioma endothelial cells(HemECs),and provide basis for the study of the mechanism of itraconazole in the treatment of hemangioma.Methods HemECs cultured in vitro were interfered with different concentrations(30μg/mL,40μg/mL,50μg/mL)of itraconazole(12 h,24 h).The morphological changes of HemECs were observed by inverted microscope,the cell viability and inhibition rate were detected by CCK-8 method,and the apoptosis rate of HemECs induced by itraconazole was detected by flow cytometry.Results Itraconazole had little effect on HemECs when the concentration of itraconazole was 5μg/mL,10μg/mL and 20μg/mL.When the concentration of itraconazole was≥30μg/mL,the effect began to be significant gradually,and there was a positive correlation between itraconazole concentration and apoptosis,and the inhibition rate of each concentration was significantly different from that of the blank group(P<0.05).The intervention time of itraconazole was also positively correlated with the inhibition of cell proliferation and apoptosis of HemECs.Conclusion Itraconazole can inhibit the growth of HemECs.The higher the concentration,the longer the intervention time,the more significant the inhibitory effect of HemECs on cell proliferation and the promotion of apoptosis.When the concentration is≥30μg/mL,the effect is gradually significant,which is positively correlated with apoptosis and inhibition of cell proliferation,and 24 h is the appropriate intervention time.
出处
《浙江临床医学》
2024年第3期333-335,339,共4页
Zhejiang Clinical Medical Journal
基金
浙江省自然科学青年基金资助项目(LQ19H280004)
浙江省中医药优秀青年人才基金项目(2018ZQ013)
浙江省台州市科技计划项目(22ywa07)
浙江省药学会科研资助项目(2016ZYY34)。
