摘要
为了评价灵芝孢子油缓解小鼠体力疲劳的作用并探究其可能机制。将144只SPF级雄性KM小鼠随机分为4组,即溶剂对照组、灵芝孢子油低、中、高剂量组,每组36只,灌胃30 d。共分为4批,分别用于负重游泳实验(Ⅰ)、肝糖原(Ⅱ)、血乳酸(Ⅳ)以及血清尿素氮、肌糖原及其他指标(腓肠肌HE染色、PAS染色和TUNEL染色)测定(Ⅲ)。结果表明,与溶剂对照组相比,灵芝孢子油中剂量组小鼠负重游泳时间极显著延长(P<0.01);灵芝孢子油各剂量组小鼠的血清尿素氮水平均极显著降低(P<0.01);各组小鼠的肝糖原和肌糖原含量无显著差异(P>0.05);灵芝孢子油各剂量组小鼠的血乳酸曲线下面积均极显著降低(P<0.01)。腓肠肌HE染色结果显示各组均未见明显病理改变。PAS染色结果显示灵芝孢子油高剂量组肌糖原阳性面积显著增加(P<0.05)。TUNEL染色结果显示灵芝孢子油低剂量组凋亡细胞阳性率显著降低(P<0.05)。因此,在本实验条件下,灵芝孢子油具有缓解体力疲劳的作用,其机制可能与减少机体糖原消耗、减轻肌细胞凋亡有关。
To evaluate the effect of Ganoderma lucidum spore oil(GLSO)on alleviating physical fatigue in mice and explore its potential mechanism.144 SPF-grade male KM mice were randomly divided into four groups:solvent control group,low-dose GLSO group,medium-dose GLSO group,and high-dose GLSO group,36 mice in each group,administered orally for 30 days.Mice were divided into four batches,which were used for measuring weight-bearing swimming time(Ⅰ),liver glycogen levels(Ⅱ),blood lactic acid levels(Ⅳ),serum urea nitrogen levels,muscle glycogen levels,and other indicators(including gastrocnemius HE staining,PAS staining,and TUNEL staining)(Ⅲ),respectively.Results showed that compared with the solvent control group,the weight-bearing swimming time of the mice in medium-dose GLSO group significantly prolonged(P<0.01);the serum urea nitrogen levels of mice in all dose groups of GLSO significantly reduced(P<0.01);the levels of liver glycogen and muscle glycogen in all groups showed no significant difference(P>0.05);the area under the curve of blood lactic acid of mice in all dose groups of GLSO significantly reduced(P<0.01).HE staining results showed no obvious pathological changes in each group.PAS staining results showed that the positive area of muscle glycogen in the high-dose GLSO group significantly increased(P<0.05).TUNEL staining results showed that the positive rate of apoptotic cells in the low-dose GLSO group significantly reduced(P<0.05).Thus,GLSO can relieve physical fatigue,and its mechanism may be related to reducing glycogen consumption and the apoptosis of gastrocnemius muscle cells.
作者
于梦淇
黄淑贞
刘雨菲
杨华
曹莉
张立实
陈锦瑶
YU Mengqi;HUANG Shuzhen;LIU Yufei;YANG Hua;CAO Li;ZHANG Lishi;CHEN Jinyao(West China School of Public Health/West China Fourth Hospital,Sichuan University,Chengdu 610041,China;Key Laboratory of Food Safety Monitoring and Risk Assessment,Chengdu 610041,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2024年第7期39-45,共7页
Food and Fermentation Industries
