摘要
氟喹诺酮类药物具有广谱高效的优点,现已广泛用于预防和治疗动物的各种感染性疾病。然而,氟喹诺酮类药物长期大量的滥用,在食品中的残留超标,导致体内耐药病原菌的产生,引起人类的耐药性问题。因此,建立快速灵敏的氟喹诺酮类药物检测方法具有重要意义。免疫分析法具有高灵敏和低成本的优势,故受到食品安全领域的广泛关注。因此,该文对氟喹诺酮类药物的抗体制备、各种免疫分析方法(包括酶联免疫吸附法、免疫层析法、荧光免疫分析法和免疫传感器)的原理、特点和应用进行总结,并对氟喹诺酮类药物免疫分析方法的发展趋势进行了展望。
Fluoroquinolones have the advantages of a broad spectrum and high efficiency,so they have been widely applied to prevent and treat various infectious diseases in animals.However,the long-term abuse of fluoroquinolones leads to excessive residues of fluoroquinolones in food,which leads to the emergence of drug-resistant pathogens in the body and causes drug-resistance problems in humans.Therefore,it is of great significance to develop a rapid and sensitive method for the detection of fluoroquinolones.Immunoassay has some merits such as high sensitivity and low cost,so it has attracted great attention in the field of food safety.Therefore,this paper summarized antibody production,the principles,characteristics,and applications of various immunoassays for the determination of fluoroquinolones,including enzyme-linked immunosorbent assay,immunochromatography assay,fluoroimmunoassay,and immunosensor.This paper also looked forward to the development trend of immunoassays for fluoroquinolones analysis in the future.
作者
王雪晴
陈秀金
李兆周
王耀
安彪
白玉冰
代明慧
陈佳琪
WANG Xueqing;CHEN Xiujin;LI Zhaozhou;WANG Yao;AN Biao;BAI Yubing;DAI Minghui;CHEN Jiaqi(College of Food and Bioengineering,Henan University of Science and Technology,Luoyang 471000,China;Henan International Joint Laboratory of Food Green Processing and Quality Safety Control,Luoyang 471000,China;National Demonstration Center for Experimental Food Processing and Safety Education,Luoyang 471000,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2024年第7期374-382,共9页
Food and Fermentation Industries
基金
国家自然科学基金项目(31701694,31702218)
洛阳市公益性行业科研专项(2202021A)
河南省青年人才托举工程项目(2020HYTP029)
河南省研究生教育改革与质量提升工程项目(HNYJS2020JD06)。
