摘要
试验旨在研究不同水平的葡萄糖氧化酶对产蛋后期海兰褐蛋鸡的产蛋性能、机体免疫力及抗氧化能力的影响。选择健康的海兰褐蛋鸡1 000只,随机分成5组,每组10个重复,每个重复20只。对照组、试验1组、试验2组、试验3组和试验4组海兰褐蛋鸡分别饲喂含有0 (对照组)、100、200、400、800 mg/kg的葡萄糖氧化酶的试验饲粮。预试期7 d,正式试验期56 d。结果表明,试验2组、试验3组和试验4组海兰褐蛋鸡的产蛋率、平均日产蛋重显著高于对照组(P<0.05),料蛋比显著低于对照组和试验1组(P<0.05)。试验3组和试验4组鸡蛋的哈夫单位显著高于对照组和试验1组(P<0.05)。试验3组和试验4组蛋鸡血清中免疫球蛋白A和免疫球蛋白M水平显著高于对照组和试验1组(P<0.05),试验2组、试验3组和试验4组蛋鸡血清中免疫球蛋白G水平显著高于对照组(P<0.05)。试验2组、试验3组和试验4组蛋鸡血清中干扰素-γ、白细胞介素-2及白细胞介素-10水平显著高于对照组(P<0.05),而肿瘤坏死因子-α水平显著低于对照组和试验1组(P<0.05)。试验2组、试验3组和试验4组蛋鸡血清中总抗氧化能力和谷胱甘肽过氧化物酶、超氧化物歧化酶活力显著高于对照组(P<0.05),试验3组和试验4组血清超氧化物歧化酶活力显著高于试验1组(P<0.05);所有葡萄糖氧化酶组血清丙二醛水平均显著低于对照组(P<0.05)。研究表明,葡萄糖氧化酶可以提高蛋鸡产蛋性能、机体免疫力及抗氧化功能,葡萄糖氧化酶的适宜添加水平为400 mg/kg。
The purpose of the study was to study the effects of different levels of glucose oxidase on the egg laying performance,immunity and antioxidant capacity of Hyline brown laying hens in the late laying period.1 000 healthy Hyline brown laying hens were selected and randomly divided into five groups,with 10 replicates per group and 20 hens per replicate.The Hyline brown laying hens in the control group,test group 1,test group 2,test group 3,and test group 4were fed with 0,100,200,400 and 800 mg/kg of glucose oxidase.The pre-test period was seven days,and the formal test period was 56 days.The results showed that the egg laying rate and average daily egg weight of Hyline brown laying hens in the test group 2,test group 3 and,test group 4 were significantly higher than those in the control group(P<0.05),and the feed-to-egg ratio was significantly lower than that in the control group and the test group 1(P<0.05).The Huff units of eggs in the test group 3 and test group 4 were significantly higher than those in the control group and test group 1(P<0.05).The serum levels of immunoglobulin A and immunoglobulin M in the test group 3 and test group 4 were significantly higher than those in the control group and test group 1(P<0.05),and the serum levels of immunoglobulin G in the test group 2,test group 3,and test group 4 were significantly higher than those in the control group(P<0.05).The serum levels of interferon-γ,interleukin-2,and interleukin-10 in the test group 2,test group 3,and test group 4 were significantly higher than those in the control group(P<0.05).The level of tumor necrosis factor-α was significantly lower than that of the control group and the test group 1(P<0.05).The activities of total antioxidant capacity,glutathione peroxidase,and superoxide dismutase in the serum of laying hens in the test group 2,test group 3 and test group 4 were significantly higher than those in the control group(P<0.05),and the serum activities of superoxide dismutase in the test group 3 and test group 4 were significantly higher than those in the group 1(P<0.05).The serum malondialdehyde level in all glucose oxidase groups was significantly lower than that in the control group(P<0.05).The study indicates that glucose oxidase can improve egg production performance,immunity,and antioxidant function of laying hens,and the appropriate addition level of glucose oxidase is 400 mg/kg.
作者
李亮
于振洋
LI Liang;YU Zhen-yang
出处
《饲料研究》
CAS
北大核心
2024年第5期43-47,共5页
Feed Research
关键词
海兰褐蛋鸡
葡萄糖氧化酶
产蛋性能
免疫
抗氧化
Hyline brown laying hen
glucose oxidase
laying performance
immunity
antioxidation