摘要
目的优选鸡血藤抗肿瘤中药复方制剂的制备工艺条件,为该验方开发成中药复方新药奠定基础。方法通过单因素试验优选水蒸气蒸馏法提取莪术中的挥发油的提取工艺;通过正交试验,以柴胡中柴胡皂苷a质量浓度,重楼中重楼皂苷Ⅰ、重楼皂苷Ⅱ、重楼皂苷Ⅶ的总量和固形物为评价指标,进行综合评分,以煎煮次数、煎煮时间、加水量为考察因素优选复方水提取的最佳工艺条件并验证。结果最佳提取工艺确定为:加14倍量水提取5 h,收集莪术挥发油蒸馏液,药渣与其余3味药加6倍量水,提取3次,每次2 h。结论通过试验优选出鸡血藤复方制剂的最佳提取工艺,该工艺条件合理且稳定可行,可为该制剂的工业生产提供实验依据。
Objective To optimize the extraction process of anti-tumor traditional Chinese medicine-Jixueteng compound preparation,and establish a foundation for the development of the new traditional Chinese medicine.Methods Volatile oil from Curcumae Rhizoma was extracted by steam distillation method,and the extraction technology was optimized by single-factor test.The extraction process of Jixueteng compound preparation was optimized by orthogonal test,with the decoction frequency,decoction time and amount of water added as the investigation factors,and with the saikosaponin A,PolyphyllinⅠ,PolyphyllinⅡ,PolyphyllinⅦcontents and solid mass as the comprehensive evaluation indicators,and then the verification test was conducted.Results The optimal preparation process was as follows:extracted 5 h with 14 times the amount of water to collect oil.The rest medical materials with herb residue were boiled thrice with 6 times of the amount of water,2 hour for each time.Conclusion The extraction process of Jixueteng compound preparation has been optimized.The process conditions are reasonable,stable and feasible,which can provide an experimental basis for the industrial production of Jixueteng formula preparation.
作者
曹颖男
张素中
毕晓黎
谢灿辉
胥爱丽
CAO Yingnan;ZHANG Suzhong;BI Xiaoli;XIE Canhui;XU Aili(Guangzhou Xinhua University,Guangzhou 510520,China;Guangdong Provincial Engineering Technology Research Institute of T.C.M,Guangzhou 510095,China;Guangdong Provincial Key Laboratory of Research and Development in Traditional Chinese Medicine,Guangzhou 510095,China;The Fifth Clinical Medical College,Guangzhou University of Traditional Chinese Medicine,Guangzhou 510405,China)
出处
《广东药科大学学报》
CAS
2024年第2期51-56,共6页
Journal of Guangdong Pharmaceutical University
基金
2021年度广东省重点建设学科科研能力提升项目(2021ZDJS143)
2021年省属科研机构稳定性支持项目(粤财科教[2021]113号)。
关键词
鸡血藤复方制剂
提取工艺
正交试验
Jixueteng compound preparation
extraction process
orthogonal test
