摘要
为了探究施用合成菌群对大豆生长和根际及根内微生物的影响,利用大豆根际促生菌,通过功能互补和功效叠加原则构建4组合成菌群(SynComs)。根据盆栽试验的促生效果选出最优菌群并取其根际土和根系分别进行宏基因组测序和16S rRNA基因测序,探究合成菌群对大豆根际土壤和根内微生物群落和功能的影响。盆栽试验表明,合成菌群SynCom 4可显著增加大豆株高、地上鲜重、根长、根鲜重和根瘤数。SynCom 4由克雷伯氏菌JP07(Klebsiella JP07)、克雷伯氏菌GD04、假单胞菌LH19(Pseudomonas LH19)、不动杆菌JP32(Acinetobacter JP32)、克雷伯氏菌PKO08、芽孢杆菌JK32(Bacillus JK32)、寡养单胞菌GD03(Stenotrophomonas GD03)和克雷伯氏菌LH13组成。宏基因组测序结果表明,接种SynCom 4提高了细菌微酸菌纲(unclassified_Acidimicrobiia)、藤黄色单胞菌属(Luteimonas)、绿屈挠菌纲(unclassified_c_Chloroflexia)、热厌氧杆状菌目(unclassified_o_Thermoanaerobacteroles)等的相对丰度,增加了参与氮代谢、磷转化、吲哚乙酸和赤霉素生物合成等功能基因的相对丰度。16S rRNA基因测序结果表明,SynCom 4增加了大豆根内细菌群落多样性,提高了黄单胞菌科(unclassified_f_Xanthomonadaceace)、食氢产水菌属(Hydrogenophaga)、根瘤菌目(unclassified_o_Rhizobiales)、黄杆菌科(norank_f_Xanthobacteraceae)等的相对丰度。PICRUSt2功能预测表明,SynCom 4增强了与氨基酸、维生素、脂质和次级代谢产物合成有关的功能。综上,宏基因组测序和16S rRNA基因测序结果表明,SynCom 4显著改变了根际和根内微生物群落的结构和组成,提高了微生物的多样性,增加了菌属和功能基因的相对丰度。施用SynCom 4有效促进了大豆生长,改变了根际和根内的细菌微生物群落结构和功能。
Plant growth-promoting rhizobacteria(PGPR)are widely used to improve soil nutrient content and promote plant growth and health.However,the growth-promoting effects of a single PGPR on plants are limited.To explore the effects of synthetic microbial communities on soybean growth,rhizosphere,and endophytic microbiota,soybean PGPR was used to construct four synthetic microbial communities(SynComs)based on functional complementarity and efficacy stacking principles.The optimal community was chosen for its growth-promoting effects,and metagenomic sequencing was performed on its rhizosphere soil,while roots underwent 16S rRNA gene sequencing.This aimed to investigate the microbial community structure and functional changes of bacteria in both the soybean rhizosphere and endosphere.Pot experiments showed that SynCom 4 significantly increased soybean plant height,aboveground fresh weight,root length,root fresh weight,and nodule number.SynCom 4 comprised Klebsiella JP07,Klebsiella GD04,Pseudomonas LH19,Acinetobacter JP32,Klebsiella PKO08,Bacillus JK32,Stenotrophomonas GD03,and Klebsiella LH13.Principal component and community composition analyses were performed using the metagenomic sequencing data,uncovering significant changes in the rhizosphere microbial community structure after treatment with SynCom 4.Ten major phyla,including Proteobacteria,Firmicutes,and Actinobacteria,were identified at the phylum level.LEfSe analysis indicated an increase in the relative abundance of taxa such as unclassified_Acidimicrobiia,Luteimonas,unclassified_c_Chloroflexia,and unclassified_o_Thermoanaerobacteroles in SynCom 4.Introducing SynCom 4 increased the relative abundance of functional genes associated with nitrogen metabolism,phosphorus transformation,and indole acetic acid,and gibberellin biosynthesis.According to 16S rRNA gene sequencing results,community composition analysis revealed that the dominant bacterial phyla in the endosphere included Proteobacteria,Firmicutes,Bacteroidetes,and Actinobacteria.Inoculation with SynCom 4 increased the diversity of the soybean endosphere bacterial community.LEfSe analysis demonstrated an increase in the relative abundance of taxa such as unclassified_f_Xanthomonadaceace,Hydrogenophaga,unclassified_o_Rhizobiales,and norank_f_Xanthobacteraceae following the application of SynCom 4.PICRUSt2 functional predictions indicated that SynCom 4 enhanced functions related to synthesizing amino acids,vitamins,lipids,and secondary metabolites.In summary,metagenomic and 16S rRNA gene sequencing results indicated that SynCom 4 significantly altered the structure and composition of the rhizosphere and endosphere microbial communities.This intervention led to increased microbial diversity and an elevated relative abundance of microbes.The application of SynCom 4 effectively promoted soybean growth and changed the structure and function of the bacterial microbial community in the rhizosphere and endosphere.
作者
周佳新
刘悦
徐伟慧
王志刚
陈文晶
胡云龙
ZHOU Jiaxin;LIU Yue;XU Weihui;WANG Zhigang;CHEN Wenjing;HU Yunlong(College of Life Science and Agroforestry,Qiqihar University/Heilongjiang Provincial Technology Innovation Center of Agromicrobial Preparation Industrialization/Heilongjiang Provincial Collaborative Innovation Center of Agrobiological Preparation Industrialization,Qiqihar 161006,China)
出处
《中国生态农业学报（中英文）》
CAS
CSCD
北大核心
2024年第4期571-581,共11页
Chinese Journal of Eco-Agriculture
基金
黑龙江省杰出青年基金项目(JQ2023D001)
黑龙江省重点研发计划项目(GA21B007)资助。
关键词
大豆
根际促生菌
合成菌群
盆栽试验
功能预测
宏基因组
Soybean
Rhizosphere-promoting bacteria
Synthetic community
Pot experiment
Functional predictions
Metagenomics
