摘要
目的 了解生殖中心就诊的育龄男性尿中二苯甲酮型紫外线吸收剂暴露与精液质量之间的关系。方法 选取2018年至2019年在广东省深圳市人民医院生殖医学中心进行精液检查的445名育龄男性人群,分别进行问卷调查及5种尿液二苯甲酮型紫外线吸收剂[包括2,4-二羟基二苯酮(2,4-dihydroxybenzophenone, BP-1)、2,2′,4,4′-四羟基二苯酮(2,2′,4,4′-tetrahydroxybenzophenone, BP-2)、二苯酮-3(benzophenone-3,BP-3)、2,2′-二羟基-4-甲氧基二苯酮(2,2′-dihydroxy-4-methoxybenzophenone, BP-8)、4-羟基二苯酮(4-hydroxybenzophenone, 4-OH-BP)]浓度的检测,采用计算机辅助精液分析系统对精液质量进行分析。依照世界卫生组织人类精液检测与处理实验手册(第五版),将精液质量分为异常组和正常组。采用多元线性回归及Logistic回归模型分析育龄男性尿中二苯甲酮型紫外线吸收剂暴露与精液质量之间的关系。结果 精液质量正常组和异常组人群的年龄、BMI、禁欲时间、吸烟、饮酒情况均无统计学差异(P>0.05)。精子浓度异常组的年龄显著高于正常组,而精子数量异常组的年龄明显低于正常组。正常组的BMI高于精子总运动率异常组,且两组间的禁欲时间差异有统计学意义(P=0.028)。在校正了年龄、BMI、禁欲天数、吸烟和饮酒情况后,多元线性回归结果显示,与最低三分位组(<0.05μg/g肌酐)相比,BP-1最高三分位组(>0.13μg/g肌酐)与精子不运动率存在正向关联(β=0.16,95%CI:0.004~0.30),与高激活精子百分率存在负向关联(β=-0.24,95%CI:-0.54~-0.01)。Logistic回归模型显示,BP-1最高三分位组(>0.13μg/g肌酐)精子前向运动率异常的风险为最低三分位组(<0.05μg/g肌酐)的1.74倍(OR=1.74,95%CI:1.03~2.93),趋势性检验具有统计学意义(P=0.029)。与最低分位组(<0.03μg/g肌酐)相比,4-OH-BP位于第二分位组(0.03~0.07μg/g肌酐)时精子前向运动率异常的风险校正OR(95%CI)值为1.91(1.13,3.24)。结论 生殖中心就诊的育龄男性人群尿液BP-1和4-OH-BP水平可能与精液质量下降有关,主要影响精子的动力学指标,而潜在机制有待于进一步探究。
Objective To understand the relationship between urinary benzophenone(BP)ultraviolet absorber exposure and se-men quality in men of reproductive age attending a fertility center.Methods A total of 445 men of reproductive age who underwent se-men examination at the Reproductive Medicine Center of Shenzhen People’s Hospital,Guangdong Province,China from 2018 to 2019 were selected for questionnaire survey and the detection of five urinary BP ultraviolet absorbers[2,4-dihydroxybenzophenone(BP-1),2,2′,4,4′-tetrahydroxybenzophenone(BP-2),benzophenone-3(BP-3),2,2′-dihydroxy-4-methoxybenzophenone(BP-8),4-hydroxy-benzophenone(4-OH-BP)].A computer-assisted semen analysis system was used to analyze semen quality.According to the World Health Organization Laboratory Manual for the Examination and Processing of Human Semen(Fifth Edition),men were assigned to groups with normal and abnormal semen quality.Multiple linear regression and logistic regression models were used to analyze the rela-tionship between urinary BP ultraviolet absorber exposure and semen quality.Results No statistical differences were found in age,body mass index,duration of abstinence,smoking,and alcohol consumption between the normal and abnormal semen quality groups(P>0.05).The age of the abnormal sperm concentration group was significantly higher than that of the normal group,while the age of the abnormal sperm count group was significantly lower than that of the normal group.Body mass index of normal group was higher than that of the total sperm motility abnormality group.The duration of abstinence was significantly different between the two groups(P=0.028).After correcting for age,body mass index,days of abstinence,smoking,and alcohol consumption,multiple linear regression results showed that compared to the lowest tertile of BP-1,the highest tertile of BP-1 was positively associated with sperm immobility(β=0.16,95%CI:0.004-0.30)and negatively associated with high percentage of activated spermatozoa(β=-0.24,95%CI:-0.54--0.01).Logistic regression model showed that the highest tertile of BP-1 was associated with a 1.74-fold risk of abnormal sperm for-ward motility compared to the lowest tertile(OR=1.74,95%CI:1.03-2.93),and the test for trend was statistically significant(P=0.029).The corrected OR(95%CI)for abnormal sperm forward motility was 1.91(1.13,3.24)for men with the second tertile of 4-OH-BP compared to those with the lowest tertile.Conclusion Urinary BP-1 and 4-OH-BP levels in the men of reproductive age atten-ding the reproductive centre may be associated with decreased semen quality.BP-1 and 4-OH-BP mainly affect the kinetic indicators of spermatozoa.The underlying mechanisms need to be further explored.
作者
鲁淇
袁冠湘
刘佩意
陈佳欣
邹子扬
吕子全
程锦泉
黄素丽
LU Qi;YUAN Guan-xiang;LIU Pei-yi;CHEN Jia-xin;ZOU Zi-yang;LYU Zi-quan;CHENG Jin-quan;HUANG Su-i(School of Public Health,Shanxi Medical University,Taiyuan 030001,China;Shenzhen Center for Disease Control and Prevention;School of Public Health,Southern Medical University)
出处
《环境卫生学杂志》
2024年第3期208-217,共10页
JOURNAL OF ENVIRONMENTAL HYGIENE
基金
国家自然科学基金面上项目(81973004)
深圳市科技创新委员会基金(JCYJ20220530155011024)。
关键词
紫外线吸收剂
精液质量
生殖健康
ultraviolet absorber
semen quality
reproductive health
