摘要
罗非鱼皮富含胶原蛋白,是一种制备生物活性肽的理想原料。以罗非鱼皮为原料,采用酶解法制备罗非鱼皮胶原酶抑制肽(Tilapia skin collagenase inhibitory peptide,TSCIP),并对其胶原酶抑制活性与金属离子结合活性的相关性进行研究,以期为罗非鱼加工副产物的高值化利用与食物蛋白源胶原酶抑制剂的研发提供参考。结果表明,罗非鱼皮经碱性蛋白酶酶解4 h后的产物具有最高的胶原酶抑制活性和锌离子(Zn^(2+))、镁离子(Mg^(2+))结合活性,并探明其主要由小分子肽(<1000 D占85.65%)组成。紫外、傅里叶变换红外光谱以及圆二色谱分析显示,TSCIP与Zn^(2+)、Mg^(2+)以及胶原酶结合后其二级结构发生了变化,β-折叠含量明显增加;Zn^(2+)、Mg^(2+)主要通过羧基氧、氨基氮原子以及羰基与TS-CIP结合,胶原酶主要通过氨基氮原子以及羰基与TSCIP结合。
Tilapia skin,rich in collagen,is an ideal raw material for preparation of bioactive peptides.To provide references for the high-value utilization of tilapia processing by-products and the development of food protein-derived collagenase inhibitors,we prepared tilapia skin collagenase inhibitory peptide(TSCIP)from tilapia skin by enzymatic hydrolysis,and studied the correlation between its collagenase inhibitory activity and metal ion binding activity.The results show that the tilapia skin product hydrolyzed by alkaline protease for 4 h had the highest collagenase inhibition activity,as well as Zn^(2+)and Mg^(2+)binding activity,and it was mainly composed of small molecular peptides(<1000 D accounting for 85.65%).Ultraviolet(UV),Fourier transform infrared(FTIR)and circular dichroism(CD)analysis show that the secondary structure of TSCIP changed after binding with Zn^(2+),Mg^(2+)and collagenase,and the β-sheet content of TSCIP increased significantly.Zn^(2+)and Mg^(2+)bind to TSCIP mainly through carboxyl oxygen,amino nitrogen atoms and carbonyl groups,while collagenase binds to TSCIP mainly through amino nitrogen atoms and carbonyl groups.
作者
鲁晨
胡晓
陈胜军
吴燕燕
黄卉
相欢
戚勃
邓建朝
LU Chen;HU Xiao;CHEN Shengjun;WU Yanyan;HUANG Hui;XIANG Huan;QI Bo;DENG Jianchao(College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China;South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences/Key Laboratory of Aquatic Product Processing,Ministry of Agriculture and Rural Affairs,Guangzhou 510300,China;Sanya Tropical Fisheries Research Institute,Sanya 572000,Hainan)
出处
《南方水产科学》
CAS
CSCD
北大核心
2024年第2期150-159,共10页
South China Fisheries Science
基金
国家重点研发计划项目(2022YFD2100905)
国家特色淡水鱼产业技术体系(CARS-46)
海南省自然科学基金(323MS123)
茂名市引进创新创业团队项目(200201095834980)
中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金资助(2023TD74)
中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金资助(2021SD06)。
关键词
罗非鱼皮
肽
胶原酶抑制活性
理化特性
Tilapia skin
Peptides
Collagenase inhititory
Physicochemical properties
