摘要
目的 探讨非小细胞肺癌(NSCLC)组织中环状核糖核酸杆状病毒IAP重复序列6(circBIRC6)、β淀粉样蛋白前体蛋白结合蛋白2(APPBP2)表达及临床预后意义。方法 收集2018年6月~2020年1月90例在河北北方学院附属第一医院行手术切除的NSCLC组织及癌旁组织,采用实时荧光定量聚合酶链式反应检测circBIRC6、APPBP2表达,并分析二者与NSCLC患者临床病理特征的关系。通过Pearson相关性分析NSCLC组织中circBIRC6与APPBP2 mRNA表达的相关性,Kaplan-Meier法绘制不同表皮生长因子受体(EGFR)基因突变、TNM分期和circBIRC6、APPBP2 mRNA表达的NSCLC患者生存曲线,多因素Cox回归分析NSCLC患者预后的影响因素。结果 与癌旁组织比较,NSCLC组织中circBIRC6、APPBP2 mRNA表达升高(P<0.05)。NSCLC组织中circBIRC6与APPBP2 mRNA表达呈正相关(r=0.817,P<0.001)。NSCLC患者不同分化程度、TNM分期、淋巴结转移组织中circBIRC6、APPBP2 mRNA表达比较有差异(P<0.05)。随访3年,90例NSCLC患者总生存率为55.56%(50/90)。EGFR基因突变阳性/阴性NSCLC患者总生存率比较无差异(P>0.05);TNM分期Ⅰ~Ⅱ期NSCLC患者总生存率高于Ⅲ期NSCLC患者(P<0.05);circBIRC6、APPBP2 mRNA高表达组生存率低于circBIRC6、APPBP2 mRNA低表达组(P<0.05)。低分化、TNM分期Ⅲ期、淋巴结转移和circBIRC6≥10.97、APPBP2 mRNA≥2.48为NSCLC患者死亡的独立危险因素[OR(95%CI)=3.586(1.080~11.909)、3.632(1.193~11.057)、3.197(1.060~9.640)、3.223(1.086~9.570)、2.767(1.022~7.492)]。结论 NSCLC组织中circBIRC6、APPBP2 mRNA高表达,与分化程度、TNM分期、淋巴结转移和预后有关。
Objective To investigate the expression of circular ribonucleic acid baculoviral IAP repeat containing 6(circBIRC6),amyloid beta precursor protein binding protein 2(APPBP2) and their clinical prognostic significance in non-small cell lung cancer(NSCLC) tissues.Methods 90 cases of surgically resected NSCLC tissues and paraneoplastic tissues from June 2018 to January 2020 at the First Hospital of Hebei North College were collected,and circBIRC6 and APPBP2 expressions were detected by real-time fluorescence quantitative polymerase chain reaction,and the relationship between them and the clinicopathological characteristics of NSCLC patients was analyzed.Pearson correlation was used to analyze the correlation between circBIRC6 and APPBP2 mRNA expression in NSCLC tissues,Kaplan-Meier method was used to plot the survival curves of NSCLC patients with different epidermal growth factor receptor(EGFR) gene mutations,TNM staging,and circBIRC6 and APPBP2 mRNA expression,and multifactorial Cox regression was used to analyze the influencing factors of prognosis of NSCLC patients.Results The expression of circBIRC6 and APPBP2 mRNA was elevated in NSCLC tissues compared with paraneoplastic tissues(P<0.05).There was a positive correlation between circBIRC6 and APPBP2 mRNA expression in NSCLC tissues(r=0.817,P<0.001).There were differences in circBIRC6 and APPBP2 mRNA expression in NSCLC patients with different degrees of differentiation,TNM stage,and lymph node metastasis tissues compared(P<0.05).At 3years follow-up,the overall survival rate of the 90 NSCLC patients was 55.56%(50/90).There was no difference in the comparison of the overall survival rate of patients with EGFR gene mutation-positive/negative NSCLC(P>0.05);the overall survival rate of patients with TNM stage Ⅰ-Ⅱ NSCLC was higher than that of patients with stageⅢ NSCLC(P<0.05);the group with high expression of circBIRC6 and APPBP2 mRNA was lower than that of the group with low expression of circBIRC6 and APPBP2 mRNA(P <0.05).Hypofractionation,TNM stage Ⅲ,lymph node metastasis and circBIRC6 ≥10.97 and APPBP2 mRNA ≥2.48 were independent risk factors for death in NSCLC patients [OR(95% CI)=3.586(1.080 to 11.909),3.632(1.193 to 11.057),3.197(1.060 to9.640),3.223(1.086 to 9.570),2.767(1.022 to 7.492)].Conclusion High expression of circBIRC6 and APPBP2 mRNA in NSCLC tissues is associated with the degree of differentiation,TNM stage,lymph node metastasis,and prognosis.
作者
陈丽萍
籍强
陈艳红
史永兴
冯平
林卫佳
项保利
赵建清
CHEN Liping;JI Qiang;CHEN Yanhong;SHI Yongxing;FENG Ping;LIN Weijia;XIANG Baoli;ZHAO Jianqing(Department of Respiratory and Critical Care Medicine,the First Affiliated Hospital of Hebei North University,Zhangjiakou,Hebei 075000,China;Department of Thoracic and Cardiovascular Surgery,the First Affiliated Hospital of Hebei North University,Zhangjiakou,Hebei 075000,China;Department of Medical Record Management,the First Affiliated Hospital of Hebei North University,Zhangjiakou,Hebei 075000,China)
出处
《临床肺科杂志》
2024年第5期727-733,共7页
Journal of Clinical Pulmonary Medicine
基金
河北省卫生健康委员会2021年度医学科学研究课题计划项目(20210266)。
