摘要
目的建立香茶菜中19种无机元素的指纹图谱,并对其中5种进行安全性评价。方法收集宿州产13批香茶菜样品,以微波消解法进行前处理,采用电感耦合等离子体质谱法建立19种无机元素的指纹图谱。采用主成分分析法对样品进行质量评价,采用单因子污染指数法及内梅罗综合指数法对其中砷,镉,汞,铅,铜元素进行安全性评价。结果钠、铝、铁,硼、钛、锰、铜、锌、钡,铬、镍、钼、铅,砷、镉、汞,镁,钾、钙质量浓度分别在0~5000 ng/mL、0~500 ng/mL、0~50 ng/mL、0~10 ng/mL及0~50μg/mL、0~200μg/mL范围内与各元素仪器响应值线性关系良好(r>0.9900),检测限为0.0003~0.300 ng/mL;精密度、重复性试验结果的RSD均小于5.0%;平均加样回收率为92.46%~102.26%,RSD为1.06%~3.19%(n=6)。13批样品中无机元素指纹图谱的相似度均大于0.920,主成分1-7的累积方差贡献率达92.871%。5种元素的污染情况均较轻微。结论13批宿州产香茶菜样品中前述5种元素的整体潜在风险较低。
Objective To establish the fingerprint of 19 inorganic elements in Herba Rabdosiae Amethystoidis,and to evaluate the safety of five elements among them.Methods Thirteen batches of Herba Rabdosiae Amethystoidis produced by Suzhou were collected,the microwave digestion was used for pretreatment of samples,and the inductively coupled plasma-mass spectrometry was used to establish the fingerprint of 19 inorganic elements.The principal component analysis was used to evaluate the quality of the samples,the single-factor pollution index and Nemero composite index methods were used for safety evaluation of the arsenic,cadmium,mercury,lead and copper.Results The linear range of sodium,aluminum and iron was 0-5000 ng/mL,that of boron,titanium,manganese,copper,zinc and barium was 0-500 ng/mL,that of chromium,nickel,molybdenum and lead was 0-50 ng/mL,that of arsenic,cadmium and mercury was 0-10 ng/mL,that of magnesium was 0-50μg/mL,and that of potassium and calcium was 0-200μg/mL(r>0.9900).The limit of detection was in the range of 0.0003 to 0.300 ng/mL.The RSDs of precision and repeatability tests were lower than 5.0%.The average recovery rate of the above 19 inorganic elements was in the range of 92.46%to 102.26%,with the RSDs in the range of 1.06%to 3.19%(n=6).The similarity of fingerprints of inorganic elements in 13 batches of samples was greater than 0.920,the cumulative variance contribution rate of principal components one to seven was 92.871%.The samples were slightly polluted with five elements.Conclusion The overall potential risk of the above five elements is relatively low in the 13 batches of Herba Rabdosiae Amethystoidis produced by Suzhou.
作者
余秀萍
汪正宇
李变变
崔灿
YU Xiuping;WANG Zhengyu;LI Bianbian;CUI Can(Suzhou Center for Food and Drug Inspection and Testing,Suzhou,Anhui,China 234000)
出处
《中国药业》
CAS
2024年第8期62-67,共6页
China Pharmaceuticals
基金
安徽省药品监督管理局科技创新项目[AHYJ-KJ-202117]
安徽省中药材标准研究课题[AHYJ-YP055-2022]。
关键词
宿州
香茶菜
电感耦合等离子体质谱法
指纹图谱
无机元素
主成分分析
风险评估
Suzhou
Herba Rabdosiae Amethystoidis
inductively coupled plasma-mass spectrometry
fingerprint
inorganic element
principal component analysis
risk assessment
