摘要
目的:探讨肺炎链球菌抗原快速检测联合痰涂片革兰染色在肺炎链球菌肺炎感染中的临床应用价值。方法:收集临床痰液标本分离得到的草绿色链球菌群、肺炎克雷伯菌、鲍曼不动杆菌、铜绿假单胞菌、金黄色葡萄球菌、嗜麦芽窄食单胞菌、流感嗜血杆菌及卡他莫拉菌共8种细菌各5株,肺炎链球菌培养阳性的痰液标本30例及痰涂片镜下疑似肺炎链球菌且合格的痰液标本40例,利用BinaxNOW试剂盒进行胶体金法肺炎链球菌抗原检测。结果:痰标本中分离得到的8种细菌经抗原检测结果均为阴性;胶体金法检测30例肺炎链球菌培养阳性的痰液标本阳性率为100%;痰涂片联合抗原快速检测阳性率高于痰培养,差异具有统计学意义(P<0.05)。结论:痰液肺炎链球菌抗原检测法具有较好的特异性,与痰培养结果有较好的一致性,且痰涂片革兰染色联合抗原快速检测在早期鉴定肺炎链球菌肺部感染中具有较高的临床应用价值。
Objective:To investigate the clinical value of streptococcus pneumoniae antigen rapid detection method combined with sputum smear gram stain in streptococcus pneumoniae pneumonia infection.Methods:Bacteria strains and sputum specimens were collected.5 strains of Streptococcus viridans group,Klebsiella pneumoniae,Acinetobacter baumannii,Pseudomonas aeruginosa,Staphylococcus aureus,Stenotrophomonas maltophilia,and Haemophilus influenzae,Moraxella catarrhalis were isolated from sputum specimens.30 sputum samples with Streptococcus pneumoniae and 40 sputum specimens suspected to be Streptococcus pneumoniae under the microscope were tested for streptococcus pneumoniae antigen using BinaxNOW kit.Results:8 kinds of bacteria isolated from sputum samples were detected as negative.The positive rate of 30 sputum specimens cultured with streptococcus pneumoniae by colloidal gold method was 100%(30/30).The positive rate of sputum smear combined antigen rapid detection was higher than that of sputum culture(P<0.05).Conclusion:Sputum antigen detection method of Streptococcus pneumoniae has good specificity and consistency with sputum culture,and sputum smear gram stain combined with antigen rapid detection has high clinical application value in early identification of streptococcus pneumoniae infection.
作者
蒋瑶
张红
祝静
廖涛
郭晓兰
谢宁
JIANG Yao;ZHANG Hong;ZHU Jing;LIAO Tao;GUO Xiao-lan;XIE Ning(Department of Clinical Laboratory,Affiliated Hospital of North Sichuan Medical College;School of Laboratory Medicine,North Sichuan Medical College,Nanchong 637000,Sichuan,China;Translational Medicine Research Center,North Sichuan Medical College,Nanchong 637000,Sichuan,China)
出处
《川北医学院学报》
CAS
2024年第4期502-505,共4页
Journal of North Sichuan Medical College
基金
四川省南充市社科研究“十四五”规划项目(NC23C130)
四川省南充市市校科技战略合作项目(20SXQT0058)。
关键词
痰液
肺炎链球菌
肺炎
胶体金法
革兰染色
Sputum
Streptococcus pneumoniae
Pneumonia
Colloidal gold method
Gram stain
