摘要
目的探讨硫酸庆大霉素介导的大鼠肠道菌群影响糖脂代谢和炎症改善高血压的关系。方法将Sprague-Dawley大鼠(8周龄)分为正常饮食组(ND组,n=11)、高脂肪饮食组(HFD组,n=13)和HFD联合硫酸庆大霉素灌胃组(GS组,n=13)。第5~13周每周监测血压,并在第13周评估胰岛素、白细胞介素(IL)-1β、IL-6、肿瘤坏死因子-α(TNF-α)、脂多糖(LPS)、总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白-胆固醇(LDL-C)、高密度脂蛋白-胆固醇(HDL-C)水平和稳态模型胰岛素抵抗指数(HOMA-IR),对来自大鼠结肠粪便的DNA标本进行宏基因组分析。结果第1周,3组血压比较差异无统计学意义(P>0.05)。第5~10周,GS组血压高于ND组,差异有统计学意义(P<0.05)。第5~13周,HFD组血压高于ND组,第10~13周,GS组血压低于HFD组,差异有统计学意义(P<0.05)。与ND组比较,HFD组TC、TG、LDL-C水平、HOMA-IR更高,HDL-C水平更低,差异有统计学意义(P<0.05)。与HFD组比较,GS组TG、LDL-C水平、HOMA-IR更低,HDL-C水平更高,差异有统计学意义(P<0.05)。HFD组LPS、IL-1β、IL-6、TNF-α水平高于ND组,而GS组低于HFD组(P<0.05)。偏最小二乘判别分析结果表明,HFD组、GS组肠道微生物群在结构上与ND组不同。家族水平结果显示,3组肠道微生物群结构发生了变化,包括24个发生明显变化的微生物群。层次分析显示,HFD组f-脱硫弧菌比例高于ND组,而GS组低于HFD组,差异有统计学意义(P<0.05)。结论口服抗生素硫酸庆大霉素可改善HFD引起的糖脂代谢异常和炎症,机制可能为通过调节肠道菌群结构、丰度及LPS水平来缓解高血压。
Objective To investigate the relationship between gentamicin sulfate mediated gut microbiota in affecting glucolipid metabolism and inflammation with hypertension improvement.Methods Sprague-Dawley rats(8-weeks-old)were divided into the normal diet group(ND,n=11),high fat diet group(HFD,n=13)and HFD combined with gentamicin sulfate gavage group(GS,n=13).Blood pressure was monitored weekly during 5-13 weeks,and the levels of insulin,IL-1β,IL-6,TNF-α,LPS,TC,TG,LDL-C,HDL-C and homeostasis model assessment of insulin resistance index(HOMA-IR)were assessed at 13 weeks.The metagenomic analyses were performed on DNA samples from rat colonic feces.Results The blood pressure in 13 weeks had no statistical difference among the three groups(P>0.05).The blood pressure during 5-10 weeks in the GS group was higher than that in the ND group with statistical difference(P<0.05).The blood pressure during 5-13 weeks in the HFD group was higher than that in the ND group,and the blood pressure during 10-13 weeks in the GS group was lower than that in the HFD with statistical difference(P<0.05).Compared with the group ND,the levels of TC,TG,LDL-C and HOMA-IR in the HFD group were higher,the HDL-C level was lower,and the differences were statistically significant(P<0.05).Compared with the HFD group,the levels of TG,LDL-C and HOMA-IR in the GS group were lower,the HDL-C level was higher,and the differences were statistically significant(P<0.05).The levels of LPS,IL-1β,IL-6 and TNF-αin the HFD group were higher than those in the ND group,while the GS group was lower than the HFD group(P<0.05).The partial least squares discriminant analysis showed that the intestinal microbiota in the HFD group and GS group was different in structure from that in the ND group.The family level results showed that the structure of the gut microbiota changed in three groups,including 24 microbiotas with significant changes.The hierarchical analysis showed that the proportion of f-desulphovibrio in the HFD group was higher than that in the ND group,while the GS group was lower the HFD group,and the differences were statistically significant(P<0.05).Conclusion Oral antibiotic gentamicin sulfate could improve the glucose and lipid metabolism abnormalities and inflammation caused by HFD and its mechanism may be to relieve hypertension by regulating intestinal flora structure,abundance and LPS level.
作者
欧阳辉
陈桂秀
熊什锐
朱兴春
OUYANG Hui;CHEN Guixiu;XIONG Shirui;ZHU Xingchun(Department of Medical Laboratory,Kaizhou District People’s Hospital,Chongqing 405400,China;Department of Cardiology,Dazhou Municipal Central Hospital,Dazhou,Sichuan 635000,China;Experimental Teaching Center of Pathobiology and Immunology,North Sichuan Medical University,Nanchong,Sichuan 637000,China)
出处
《重庆医学》
CAS
2024年第8期1126-1131,共6页
Chongqing medicine
基金
重庆市开州区科技局科技计划项目(2019-J-17)
四川省南充市科技战略合作项目(18SXHZ0436)。
关键词
硫酸庆大霉素
肠道微生物群
胰岛素抵抗
炎症
高血压
gentamicin sulfate
intestinal microbiota
insulin resistance
inflammation
hypertension
