摘要
ECT结构域蛋白家族作为一种重要的转录后调控因子,在调控m6A修饰中发挥重要功能,在植物正常生长发育和响应逆境胁迫时的基因表达调控中具有重要作用。为了研究ECT结构域蛋白家族在玉米(Zea mays L.)非生物胁迫下的生长发育与胁迫响应中的功能,本研究采用生物信息学方法鉴定到玉米ECT家族成员22个,并分析其序列和结构特征、染色体分布、启动子顺式作用元件、GO富集、蛋白质互作网络和系统发育进化关系,通过实时荧光定量PCR(qRT-PCR)分析了玉米ZmECTs在冷胁迫以及不同激素处理下的表达模式。结果表明,玉米ECT家族成员分布在10条染色体上,编码的蛋白质所含氨基酸残基数目为119~748 aa,相对分子量(MW)范围为13412.17~81823.70 Da,等电点(pI)范围为5.43~8.82,大部分蛋白定位在细胞核。在ZmECT家族中共鉴定到10个motif。顺式作用元件分析结果表明,ZmECT家族成员启动子包含多个与胁迫、激素和生长发育相关的响应元件。GO富集分析结果显示,ZmECT家族成员可能参与mRNA的剪接和维持RNA稳定性。蛋白质互作网络预测,ZmECT6是该家族蛋白的核心成员。系统进化树显示,ZmECT家族成员分为4组。qRT-PCR结果显示,经不同激素处理后,ZmECT家族成员表现出复杂的响应模式,部分ZmECT家族成员响应冷胁迫。本研究结果为玉米ECT基因后续的生物学功能解析及分子机制研究提供了参考。
ECT domain protein family,an important post-transcriptional regulator,participates in the regulation of m6A modification,and plays an important role in regulating the gene expression level of plants under normal and stress conditions and maintaining their normal growth and development.To investigate the function of ECT domain protein family in the growth and development of maize(Zea mays L.)under abiotic stress and stress response,in this study,22 members of maize ECT family were identified by bioinformatics,and their sequences and structural characteristics,chromosome distribution,promoter cis-acting elements,GO enrichment,protein-protein interaction networks and phylogenetic evolution were analyzed.The expression patterns of maize ZmECTs were analyzed by quantitative real-time PCR(qRT-PCR)under cold stress and different hormone treatments.Result showed that the members of maize ECT family were distributed on 10 chromosomes,the encoded proteins ranged from 119 to 748 aa,the relative molecular weight(MW)ranged from 13412.17 to 81823.70 Da,and the isoelectric point(pI)ranged from 5.43 to 8.82.Most of the proteins are localized in the nucleus.10 motifs were identified in ZmECT family.The analysis of cis-acting elements showed that the promoters of ZmECT family members contained multiple response elements related to stresses,hormones,growth and development.GO enrichment analysis showed that ZmECT family members may participate in mRNA splicing and maintaining RNA stability.Protein-protein interaction networks predicted that ZmECT6 was the core member of this family proteins.The phylogenetic tree showed that the ZmECT family members were divided into 4 groups.qRT-PCR results showed that ZmECT family members presented complex response patterns after different hormone treatments.In addition,some ZmECT family members could respond to cold stress.The above results can provide reference for the subsequent functional analysis of maize ECT genes and the study of abiotic stress,and also provide available gene resources for breeding excellent resistant maize varieties.
作者
徐可丽
周美君
郭怡婷
吉虹菁
黄敏
李曼菲
杜何为
刘睿
XU Keli;ZHOU Meijun;GUO Yiting;JI Hongjing;HUANG Min;LI Manfei;DU Hewei;LIU Rui(School of Life Sciences,Yangtze University,Jingzhou,Hubei 434000)
出处
《核农学报》
CAS
CSCD
北大核心
2024年第6期1035-1047,共13页
Journal of Nuclear Agricultural Sciences
基金
国家自然科学基金面上项目(32072069)
湖北省自然科学基金面上项目(2022CFB183)
创新群体项目(2022CFA030)。
