摘要
【目的】探究盐胁迫下高粱根系微生物群落结构变化,以及高粱根系微生物群落网络特征。【方法】以2种耐盐性不同的高粱品种河农16(盐敏感)和高粱蔗(耐盐)为试验材料,通过盆栽种植,不同盐胁迫处理,利用16S扩增子测序技术对其根系微生物组进行高通量测序。【结果】随着盐胁迫的加剧,高粱根系的总酚和总黄酮含量逐渐增加,适宜的盐胁迫通过诱导酚酸类化合物的合成来提高高粱的耐盐性。高粱根际土壤优势菌门为变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、酸杆菌门(Acidobacteria)、拟杆菌门(Bacteroidetes)和绿弯菌门(Chloroflexi)。盐胁迫条件下Proteobacterisa、Bacteroidetes和Pseudomonas的相对丰度随盐胁迫的加剧显著升高。盐胁迫下高粱根际细菌组成受生育时期的影响较小,高粱根际细菌的群落结构均随盐胁迫程度的加剧而变迁。加权基因共表达相关网络分析(weighted gene co-expression network analysis,WGCNA)鉴定了12个基因共表达模块,其中pink模块与盐胁迫显著正相关,greenyellow模块与生育时期和盐处理后的根系总酚、总黄酮含量显著正相关。低盐土壤细菌共现网络比高盐土壤更为复杂,表现为节点和连接更多。鉴定出13个网络关键OTUs,OTU8480、OTU6866、OTU3247和OTU3499是S0网络中的关键OTUs,S3网络中为OTU6895、OTU4206、OTU6470、OTU1810和OTU4916,S7网络以OTU4217、OTU8426、OTU4847和OTU6066为关键类群。【结论】盐胁迫下高粱根系微生物的多样性发生改变,共现网络更为复杂,OTUs间联系更为紧密。
【Objective】This study aims to investigate the changes in the bacterial community structure of sorghum roots under salt stress,as well as the characteristics of the rhizosphere bacterial co-occurrence network structure.【Method】Two sorghum varieties,HN16(saltsensitive)and GLZ(salt-tolerant),with varying levels of salt tolerance were cultivated in pots and subjected to salt stress.The root microbiome of these plants was then analyzed using 16S amplicon sequencing technology.【Result】With the aggravation of salt stress,the contents of total phenols and flavonoids in the sorghum roots gradually increased,and the appropriate salt stress induced the synthesis of phenolic acids to improve the salt tolerance of sorghum.The results of the microbial community structure analysis showed that Proteobacteria,Actinobacteria,Acidobacteria,Bacteroidetes and Chloroflexi were the dominant phyla in sorghum rhizosphere soils.Comparative analysis of samples showed that the bacterial composition of sorghum rhizosphere under salt stress was less affected by growth stage,and the bacterial community structure of sorghum rhizosphere changed with the aggravation of salt stress.Through WGCNA analysis of all filters OTUs,a total of 12 gene co-expression module were identified,pink module had significantly positive correlation with salinity and greenyellow module had significantly positive correlation with period and the contents of total phenols and flavonoids after salt treatment.The bacterial co-occurrence network was more complex in low salt stress soils than in higher salt stress soils,reflected by more nodes and more edges.The 13 network key OTUs were identified,OTU8480,OTU6866,OTU3247 and OTU3499 was the keyOTUs in S0 network;OTU6895,OTU4206,OTU6470,OTU1810 and OTU4916 in S3 network;OTU4217,OTU8426,OTU4847,and OTU6066 were the key OTUs in S7 network.【Conclusion】The composition of rhizosphere bacteria in both varieties of sorghum has been modified in response to salt stress.After salt stress,with more complex co-occurrence network and tighter connections between OTUs of rhizosphere soil bacterial communities.
作者
高玉坤
张建东
杨溥原
陈东明
王志博
田颐瑾
Zakey Eldinn.E.A.Khlid
崔江慧
常金华
GAO Yu-kun;ZHANG Jian-dong;YANG Pu-yuan;CHEN Dong-ming;WANG Zhi-bo;TIAN Yi-jin;Zakey EldinnEAKhlid;CUI Jiang-hui;CHANG Jin-hua(College of Agronomy,Hebei Agricultural University,North China Key Laboratory for Crop Germplasm Resources of Education Ministry,Baoding 071000)
出处
《生物技术通报》
CAS
CSCD
北大核心
2024年第4期203-216,共14页
Biotechnology Bulletin
基金
河北省重点研发计划(21326305D)
河北省高等学校科学技术研究项目(ZD2020309)。
关键词
高粱
根际
微生物群落结构
盐胁迫
sorghum
rhizosphere
microbial community structure
salt stress
