摘要
目的:基于网络药理学探讨益气化痰活血方治疗甲状腺功能减退症的作用机制。方法:采用中药系统药理学数据库与分析平台(TCMSP)、化学专业数据库、本草组鉴平台筛选出益气化痰活血方的活性成分,利用Swiss Target Prediction数据库预测活性成分的潜在靶点。利用Gene Cards数据库、Dis Ge NET数据库和在线人类孟德尔遗传数据库(OMIM)检索甲状腺功能减退症的相关靶点。利用STRING数据库对药物与疾病交集靶点进行蛋白质-蛋白质相互作用网络构建,获得益气化痰活血方治疗甲状腺功能减退症的关键靶点。利用Metascape对关键靶点进行京都基因与基因组百科全书(KEGG)信号通路与基因本体论(GO)功能富集分析。结果:得到药效成分46个、药物靶点266个、甲状腺功能减退症靶点1 294个,最终获得关键靶点69个、KEGG通路2条。益气化痰活血方治疗甲状腺功能减退症主要成分为槲皮素、β-谷甾醇、山柰酚等,关键靶点为白细胞介素(Interleukin,IL)-6、IL-10、肿瘤坏死因子(Tumor Necrosis Factor,TNF)、丝氨酸/苏氨酸蛋白激酶(Akt Serine/Threonine Kinase,Akt)、血管内皮生长因子A(Vascular Endothelial Growth Factor A,VEGFA),关键的信号通路可能包括磷脂酰肌醇3激酶-蛋白激酶B(Phosphatidylinositol 3-kinase-Akt,PI3K-Akt)信号通路、晚期糖基化终末产物-糖基化终末产物受体(AGE-RAGE)信号通路等。结论:本研究初步揭示了益气化痰活血方多成分、多靶点、多途径治疗甲状腺功能减退症的机制,为益气化痰活血方的临床开发利用提供了依据。
Objective:To explore the mechanism of the Yiqi Huatan Huoxue prescription(益气化痰活血方)on hypothyroidism based on network pharmacology.Methods:The active ingredients of the Yiqi Huatan Huoxue prescription were screened out by TCMSP,Chemical Professional Database,HERB,and the potential targets of active ingredients were predicted by SwissTargetPrediction database.GeneCards database,DisGeNET database and OMIM database were retrieue to search for the related targets of hypothyroidism.By STRING database,the protein-protein interaction network of the intersection target of drugs and diseases was constructed to obtain the key target of the Yiqi Huatan Huoxue prescription for treating hypothyroidism.KEGG signal pathway and GO function enrichment analyses of key targets were performed by Metascape.Results:A total of 46 active ingredients,266 drug targets,and 1294 targets for hypothyroidism were obtained,ultimately resulting in 69 key targets and 2 KEGG pathways.The main component of the recipe were quercetin,β-sitosterol,kaempferol,and other key targets were IL-6,IL-10,TNF,PKB,VEGFA.The key pathways might include PI3K-Akt signaling pathway,AGE-RAGE signaling pathway and so on.Conclusion:The mechanism of multi-component,multitarget and multi-pathway of the Yiqi Huatan Huoxue prescription on hypothyroidism was initially revealed in this study,which provided a basis for the clinical development and utilization of the Yiqi Huatan Huoxue prescription.
出处
《中医临床研究》
2024年第10期69-75,共7页
Clinical Journal Of Chinese Medicine
基金
国家自然基金面上项目(82274455)
国家自然科学基金青年基金项目(82104805)
辽宁省教育厅基础研究项目(L201923)
沈阳市中青年科技创新人才支持计划项目(RC200370)。
