摘要
建立了一种木质纤维素的碱催化甘油水溶液预处理方法以期选择性分离组分和提高底物的可酶解性。实验确定了碱催化甘油水溶液预处理甘蔗渣的条件为:温度180℃、NaOH添加量7%、反应时间45 min以及甘油水溶液浓度80%。在该条件下,甘蔗渣的纤维素和半纤维素保留率分别为93.0%和83.4%,而木质素脱除率接近80%,达到了理想的组分分离效果。预处理后底物(20 g/L)在CTec2酶载量10 FPU/g干基下水解72 h酶解率为79.6%,表明预处理后底物具有较好的可酶解性。利用现代分析技术解析了甘蔗渣预处理前后的组成结构演变规律,初步探明了碱催化甘油水溶液预处理有效提升生物质原料可酶解性的原因。
This study introduced an alkali-catalyzed aqueous glycerol pretreatment for fractionation of sugarcane bagasse to improve the enzymatic hydrolysability.The pretreatment conditions were optimized at 180°C,7%NaOH addition,45 min,and 80%glycerol in an aqueous solution.The pretreatment led to 93.0%and 83.4%cellulose and hemicellulose retention,respectively,while 80%of the delignification indicated a satisfactory component fractionation.Subsequently,the enzymatic hydrolysis of the pretreated substrate(20 g/L)was 79.6%at the hydrolytic condition of 10 FPU/g-dry substrate(CTec 2)for 72 h,meaning that the pretreated substrate was capable of good enzymatic hydrolysability.The structural evolution of sugarcane bagasse before and after the pretreatment was analyzed by modern analytical techniques,and the reason for the good enzymatic hydrolysability of lignocellulose substrate contributed by alkali-catalyzed aqueous glycerol pretreatment was preliminarily made.
作者
王晨
宋国杰
孙海彦
闫俊书
宦海琳
窦少华
孙付保
WANG Chen;SONG Guojie;SUN Haiyan;YAN Junshu;HUAN Hailin;DOU Shaohua;SUN Fubao(School of Biotechnology,Jiangnan University,Wuxi 214122,Jiangsu,China;Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China;Institute of Animal Science,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;College of Life and Health,Dalian University,Dalian 116622,Liaoning,China)
出处
《新能源进展》
CSCD
北大核心
2024年第2期209-215,共7页
Advances in New and Renewable Energy
基金
国家自然科学基金面上项目(22278189)
科技部国家重点研发计划专项项目(2019YFE0114600)
财政部和农业农村部国家现代农业产业技术体系项目(CARS-11-HNSHY)。
关键词
木质纤维素生物质
甘油水溶液
组分分离
酶解糖化
可发酵性糖
lignocellulosic biomass
aqueous glycerol solution
component fractionation
enzymatic hydrolysis and saccharification
fermentable sugar
