摘要
本文建立了气相色谱法(GC)检测草莓中17种有机氯、拟除虫菊酯类农药残留的方法。样品通过乙腈-乙酸(体积比为99∶1)溶液提取,盐析和净化后,经DB-17色谱柱(30.00 m×0.25 mm×0.25μm)分离,气相色谱-电子捕获检测器(GC-ECD)定量检测。结果表明,有机氯、拟除虫菊酯类农药在0.01~1.00μg/mL范围内线性关系良好,相关系数(R2)均在0.9950以上,定量限在0.3~0.9μg/kg;对样品进行0.05 mg/kg、0.10 mg/kg、0.50 mg/kg3个浓度加标回收试验,总加标回收率为88.22%~113.39%,相对标准偏差为0.19%~5.20%,满足方法学要求。该方法简单、快速、准确、经济,适用于草莓中有机氯、拟除虫菊酯类农药的定量分析。
This paper established a gas chromatography(GC)method for the detection of 17 organochlorine and pyrethroid pesticide residues in strawberries.The sample was extracted with acetonitrile-acetic acid(volume ratio of 99∶1)and then subjected to salting out and purification treatment.It was passed through a DB-17 chromatographic column(30.00 m×0.25 mm×0.25μm)for separation,and quantitatively detected by gas chromatography-electron capture detector(GC-ECD).The results showed that the linear relationship between organochlorine and pyrethroid pesticides was good within the range of 0.01 to 1.00μg/mL,with correlation coefficients(R2)above 0.9950,and the limit of quantification was between 0.3 and 0.9μg/kg.The samples were subjected to three concentration spiked recovery experiments of 0.05 mg/kg,0.10 mg/kg and 0.50 mg/kg,with total spiked recovery rates ranging from 88.22%to 113.39%and relative standard deviation ranging from 0.19%to 5.20%,which could meet the methodological requirements.This method was simple,fast,accurate,and economical,which was suitable for the quantitative analysis of organochlorine and pyrethroid pesticides in strawberries.
作者
张少英
夏慧
ZHANG Shaoying;XIA Hui(Huzhou Agricultural Product Quality and Safety Center,Huzhou Zhejiang 313000)
出处
《现代农业科技》
2024年第9期167-172,共6页
Modern Agricultural Science and Technology
关键词
气相色谱
农药残留
有机氯
拟除虫菊酯
检测
草莓
gas chromatography,pesticide residue
organochlorine
pyrethroid
determination
strawberry
