摘要
为降低甘薯(Dioscorea esculenta(Lour.)Burkill)组培苗快繁培养的生产成本,提高工厂化育苗的经济效益,在MS基本培养基的基础上,通过简化培养基组成、以自来水代替去离子水、以白砂糖代替蔗糖等降本措施,研究了连续继代培养下培养基组成简化或替代措施对甘薯组培苗生长的影响。结果表明,无论是初代培养还是继代培养,以白砂糖代替蔗糖,对甘薯组培苗生长无显著影响,但去除培养基中的微量元素、有机物质或以自来水代替去离子水,均对组培苗产生不利影响,导致株高降低、增殖系数减小、根系生长变差,且这种不利影响在经历长时间的继代培养后进一步加剧,组培苗生长更差。因此,在甘薯组培苗的工厂化生产中,可以用白砂糖代替蔗糖,降低组培生产成本,而其他降本措施不宜采用。
In order to reduce the production cost of rapid propagation of sweet potato(Dioscorea esculenta(Lour.)Burgill)tissue cul⁃ture seedlings and improve the economic benefits of factory seedling cultivation,on the basis of MS basic culture medium,the effects of simplifying or replacing the composition of culture medium on the growth of sweet potato tissue culture seedlings under continuous subculture were studied through cost reduction measures such as simplifying the composition of culture medium,replacing deionized water with tap water,and replacing sucrose with granulated sugar.The results showed that using white sugar instead of sucrose had no significant effect on the growth of sweet potato tissue cultured seedlings,whether in primary or secondary culture.However,removing trace elements and organic matter from the culture medium,or using tap water instead of deionized water,all had adverse effects on tissue culture seedlings,leading to a decrease in plant height,a decrease in proliferation coefficient,and poorer root growth,and this adverse effect was further exacerbated after prolonged subculture,resulting in poorer growth of tissue cultured seedlings.Therefore,in the factory production of sweet potato tissue culture seedlings,white sugar could be used instead of sucrose to reduce the production cost of tissue culture,while other cost reduction measures were not suitable.
作者
赵元增
王永刚
陈丹
ZHAO Yuan-zeng;WANG Yong-gang;CHEN Dan(School of Life Sciences,Henan Institute of Science and Technology,Xinxiang 453003,Henan,China)
出处
《湖北农业科学》
2024年第4期179-184,190,共7页
Hubei Agricultural Sciences
基金
河南省重点研发与推广专项(202102110035)。
