摘要
[目的]为筛选出对澳洲坚果新害虫——脊胸露尾甲成虫和幼虫灵敏的生物活性测定方法。[方法]以甲氨基阿维菌素苯甲酸盐为供试药剂,分别采用浸虫法、饲喂法和药膜法开展了生物活性测定试验。[结果]处理24 h后,浸虫法对成虫的生物活性最高(LC_(50)值为28.48 mg/L),显著高于药膜法(LC_(50)值为206.81 mg/L)和饲喂法(LC_(50)值为343.80 mg/L);饲喂法对2龄幼虫的生物活性最高(LC_(50)值为44.30 mg/L),显著高于药膜法(LC_(50)值为144.81 mg/L)和浸虫法(LC_(50)值为142.27 mg/L)。[结论]浸虫法可用于成虫的生物活性测定、饲喂法可用于幼虫的生物活性测定,该结论可为脊胸露尾甲成虫及幼虫的生物活性测定方法的选择提供参考。
[Aims]This study aims to screen sensitive methods of bioassay for adults and larvae of Carpophilus dimidiatus,a new pest to macadamia nut.[Methods]The experiment was carried out using emamectin benzoate as tested insecticide by 3 methods,namely,immersion method,feeding method and insecticide-membrane method.[Results]24 Hours after treatment,the immersion method had the best activity to adults of C.dimidiatus with an LC_(50) of 28.48 mg/L,which was significantly higher than insecticide-membrane method with an LC_(50) of 206.81 mg/L and feeding method with an LC_(50) of 343.80 mg/L.The feeding method had the best activity to 2nd instar larvae of C.dimidiatus with an LC_(50) of 44.30 mg/L,which was significantly higher than insecticide-membrane method with an LC_(50) of 144.81 mg/L and immersion method with an LC_(50) of 142.27 mg/L.[Conclusions]The Immersion method can be used to test activity of insecticides against adults of C.dimidiatus,and feeding method can be used against larvae of C.dimidiatus.The conclusion provides a reference for selection of sensitive method to determine the activity of insecticides against adults and larvae of C.dimidiatus.
作者
许丽月
吴忠华
朱国渊
张永科
王进强
贺熙勇
XU Liyue;WU Zhonghua;ZHU Guoyuan;ZHANG Yongke;WANG Jinqiang;HE Xiyong(Yunnan Macadamia Agricultural Engineering Research Center,Yunnan Institute of Tropical Crops,Jinghong 666100,Yunnan,China)
出处
《农药》
CAS
CSCD
北大核心
2024年第4期300-303,共4页
Agrochemicals
基金
云南省盈江县澳洲坚果产业科技特派团(202104BI090004)
云南省热带作物科技创新体系建设专项(RF2024-7)
云南省热带作物科学研究所青年成长基金(QNCZ2020-10)
兴滇英才支持计划。
