摘要
目的:建立高效液相色谱-串联质谱法(High Performance Liquid Chromatography-Tandem Mass Spectrometry,HPLC-MS/MS)测定食糖中4种着色剂的分析方法。方法:样品经80%甲醇超声提取,再用PWA-2固相萃取柱净化后,经VP-ODS C_(18)柱(2.0 mm×150 mm,5.0μm)分离,以乙酸铵和甲醇为流动相,梯度洗脱,用HPLC-MS/MS检测。结果:4种化合物在考察范围内线性良好,相关系数均大于0.999;加标回收率在85.36%~102.30%,RSD为0.42%~2.72%;检出限为2~5μg·kg^(-1)。结论:该方法灵敏度高、分辨率高、分析速度快,适用于食糖中合成着色剂的快速筛查和定量分析。
Objective:To establish an analytical method for the determination of four colorants in sugar using high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).Method:The sample was ultrasonically extracted with 80%methanol,purified with a PWA-2 solid phase extraction column,and then separated on a VP-ODS C_(18) column(2.0 mm×150 mm,5.0μm)with a gradient elution using ammonium acetate and methanol as the mobile phases,and detected by HPLC-MS/MS.Result:The four compounds had good linearity within the investigation range,and the correlation coefficients were all greater than 0.999;the standard recovery rate was 85.36%~102.30%,and the RSD was 0.42%~2.72%;the detection limit was 2~5μg·kg^(-1).Conclusion:This method has the characteristics of high sensitivity,resolution and fast analysis speed,and is suitable for rapid screening and quantitative analysis of synthetic colorants in sugar.
作者
陈艳
张丽
冉艳瑞
黄立
万静
马艳红
CHEN Yan;ZHANG Li;RAN Yanrui;HUANG Li;WAN Jing;MA Yanhong(Kunming Food and Drug Inspection Institute,Kunming 650000,China)
出处
《食品安全导刊》
2024年第10期67-69,共3页
China Food Safety Magazine
关键词
食糖
高效液相色谱-串联质谱法
合成着色剂
快速测定
sugar
high performance liquid chromatography-tandem mass spectrometry
synthetic colorant
rapid determination
