摘要
建立环黄杨碱D含量测定的反相离子对色谱法。色谱柱Xtimate C_(18)(250 mm×4.6 mm, 5.0μm),以[0.01 mol·L^(-1)磷酸二氢钾溶液与0.01 mol·L^(-1)庚烷磺酸钠溶液等量混合(含有0.2%的三乙胺,使用磷酸调节pH值至3.5)]-乙腈(78∶22,V/V)作为流动相,流速1.0 mL·min^(-1),检测波长208 nm,柱温35℃。环黄杨碱D平均回收率(n=6)结果为100.6%,RSD为0.88%;检测限和定量限分别为0.030μg、0.080μg。本法结果准确,重复性良好,可用于环黄杨碱D含量测定。
An ion-pairing reversed-phase high performance liquid chromatography(RP-HPLC)method for determination of the concentration of cyclobuxine D in active pharmaceutical ingredient(API)was established.RP-HPLC was performed on an Xtimate C_(18) chromatographic(4.6 mm×250 mm,5.0μm)column.The mixed solution(a mixture of equal volume of 0.01 mol·L-1 potassium dihydrogen phosphate solution and 0.01 mol·L^(-1 )solution of sodium heptanesulfonate solution,containing 0.2%triethylamine and finally adjusted to pH 3.5 with phosphoric acid)-acetonitrile(78∶22,V/V)as the mobile phase.The flow rate was 1.0 mL·min^(-1),the detection wavelength was at 208 nm,and the column temperature was 35℃.The average recoveries(n=6)of the cyclobuxine D was 100.6%with RSD was 0.88%.Limits of quantity and detection were 0.030μg and 0.080μg,respectively.This method with good accuracy and repeatability can be used for the determination of content of cyclobuxine D.
作者
林春霞
袁倩
金祥飞
沈卫阳
LIN Chun-xia;YUAN Qian;JIN Xiang-fei;SHEN Wei-yang(College of Sciences,China Pharmaceutical University,Jiangsu Nanjing 210009,China)
出处
《广州化工》
CAS
2023年第24期49-51,共3页
GuangZhou Chemical Industry
