摘要
降低木质纤维素生物炼制的用酶成本,对推动木质纤维素的绿色利用至关重要。作为纤维素水解过程中的限速酶,β-葡萄糖苷酶酶解活性受高浓度葡萄糖的胁迫抑制。目前,挖掘和突变耐糖β-葡萄糖苷酶的基因是解决这一问题的重要策略。本综述总结了近年来在耐糖β-葡萄糖苷酶基因的挖掘、酶耐糖分子机制的分析、旨在提高酶耐糖性能的酶基因突变以及基于β-葡萄糖苷酶基因的微生物菌株基因工程改造等方面所取得的进展,并进行了展望。本综述为构建高产耐糖β-葡萄糖苷酶的基因工程菌株提供参考,这对推动木质纤维素生物炼制工业化进程具有重要意义。
Reducing enzyme cost used in lignocellulosic biorefineries is important to promote the green utilization of lignocellulose.β-glucosidase,a rate-limiting enzyme during enzymatic hydrolysis of cellulose,is inhibited by a high concentration of glucose.Mining and mutating the genes of glucose-tolerantβ-glucosidases are important strategies.This paper summarizes the progress made in recent years in mining glucose-tolerantβ-glucosidase genes,analyzing the molecular mechanism of enzyme tolerance to glucose,mutation ofβ-glucosidase gene to improve the glucose-tolerance,and genetic engineering of modified microbial strains based onβ-glucosidase genes,and then perspectives.This paper will provide references for constructing genetically engineered strains with a high yield of glucose-tolerantβ-glucosidase,which is of great significance to the development of lignocellulosic biorefineries.
作者
张婷
宁远妮
冯家勋
赵帅
ZHANG Ting;NING Yuanni;FENG Jiaxun;ZHAO Shuai(College of Food and Quality Engineering,Nanning University,Nanning,530200;College of Life Science and Technology,Guangxi University,Nanning,530004)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2024年第3期377-386,共10页
Genomics and Applied Biology
基金
2023年度广西高校中青年教师科研基础能力提升项目(2023KY1871)
2023年南宁市“邕江计划”青年人才专项(RC20230202)共同资助。
