摘要
目的:探究组织线粒体DNA(mitochondrial DNA,mtDNA)含量和结直肠癌预后相关性。方法:选取117例结直肠癌患者并收集临床病理资料。运用RT-qPCR检测患者癌组织与癌旁组织mtDNA含量,探究mtDNA含量与各项预后指标的相关性。绘制受试者工作特征(receiver operating characteristic,ROC)曲线,根据截断值区分患者并绘制无病生存期(disease free survival,DFS)曲线。单因素和多因素Cox回归分析探究术后DFS相关的危险因素。结果:与癌旁组织相比,癌组织mtDNA含量差异无统计学意义(P=0.432);低mtDNA含量与肿瘤位于结肠、低分化、TNM分期差、淋巴结转移相关(P <0.05)。ROC曲线提示mtDNA含量为500.699可作为截断值。单因素和多因素分析显示,mtDNA含量低于500.699(HR=4.285,95%CI:1.938~9.475)、肿瘤低分化(HR=2.886,95%CI:1.428~5.835)是与DFS相关的独立危险因素。结论:结直肠癌患者中,组织mtDNA含量与临床病理特征有关,低mtDNA含量是患者预后相关的独立危险因素。
Objective:To investigate the prognostic correlation between mitochondrial DNA(mtDNA)content and colorectal cancer.Methods:Clinical and pathological data were collected from 117 patients with colorectal cancer.RT-qPCR was used to determine the mtDNA content of cancer tissues and paracancerous tissues of patients,and the correlation between mtDNA content and various prognostic indicators was investigated.Receiver operating characteristic(ROC)curves were plotted,and the cut-off value was determined to distinguish patients,and disease free survival(DFS)curves were plotted accordingly.Univariate and multivariate Cox regression analyses were conducted to explore the risk factors related to postoperative DFS.Results:There was no significant difference in mtDNA content between cancer tissues and paracancerous tissues(P=0.432);low mtDNA content was associated with tumor location in the colon,poor differentiation,advanced TNM stage,and lymph node metastasis(P<0.05).The ROC curve indicated a cut-off value of 500.699 for mtDNA content.Univariate and multivariate Cox analyses showed that mtDNA content lower than 500.699(HR=4.285,95%CI:1.938-9.475)and poorly differentiated tumors(HR=2.886,95%CI:1.428-5.835)were independent risk factors associated with DFS.Conclusion:In patients with colorectal cancer,tissue mtDNA content is associated with clinical and pathological characteristics,and low mtDNA content is an independent risk factor for patient prognosis.
作者
杜杨斌
郭文文
何震宇
DU Yangbin;GUO Wenwen;HE Zhenyu(Department of General Surgery,the Second Affiliated Hospital of Nanjing Medical University,Nanjing 210011,China;Department of Pathology,the Second Affiliated Hospital of Nanjing Medical University,Nanjing 210011,China)
出处
《南京医科大学学报（自然科学版）》
CAS
北大核心
2024年第5期681-687,共7页
Journal of Nanjing Medical University(Natural Sciences)
基金
南京科技发展计划项目(2018SC512012)。
关键词
线粒体DNA
结直肠癌
预后
肿瘤复发
无病生存期
mitochondrial DNA
colorectal cancer prognosis
tumor recurrence
disease-free survival
