摘要
为从沙棘中筛选生防内生真菌,从健康沙棘叶片和果实中采用组织匀浆法分离内生真菌,从中筛选具有拮抗活性的菌株,对其进行形态学和分子生物学鉴定,并对活性菌株进行产纤维素酶活性分析.从97株内生真菌中筛选得到16株对8种植物病原真菌具有较好抑菌作用的菌株,其中菌株SJ-83和SJ-116对8种病菌的抑制作用最佳.对16株活性内生真菌进行鉴定,发现菌株SJ-93产生具有横纵隔膜、呈褐色的分生孢子,为链格孢属真菌;菌株SJ-83菌丝棉絮状,浅绿褐色,产生褐色具横隔的分生孢子,为平脐蠕孢属真菌.采用透明圈法在刚果红培养基上进行产纤维素酶活性测定发现,菌株SJ-116产纤维素酶的活性最高,透明圈半径达1.6 cm.研究结果为后续开发沙棘生防内生真菌制剂提供基础材料.
In order to screen biocontrol endophytic fungi from Hippophae rhamnoides L.,endophytic fungi were isolated from healthy H.rhamnoides L.leaves and fruits by tissue homogenization method.The strains with antagonistic activity were screened,identified by morphology and molecular biology,and the cellulase activity of the active strains was analyzed.A total of 16 strains with good inhibitory effects on 8 plant pathogenic fungi was screened from 97 endophytic fungi,among which strains SJ-83 and SJ-116 had the best inhibitory effects on 8 pathogens.The morphological characteristics of 16 strains of endophytic fungi were observed.It was found that strain SJ-93 produced brown conidia with transverse and longitudinal membrane,which was identified as Alternaria.The strain SJ-83 had flocculent,light green-brown mycelium,and produced brown transversely septate conidia,which was a fungus of the genus Bipolaris.The cellulase activity was determined on Congo red medium by transparent circle method.The results showed that the cellulase activity of strain SJ-116 was the highest,and the radius of transparent circle was 1.6 cm.The results of this study provided basic materials for the subsequent development of biocontrol endophytic fungi for H.rhamnoides L.
作者
孔天赐
张海艳
白发莹
马学青
祁鹤兴
KONG Tianci;ZHANG Haiyan;BAI Faying;MA Xueqing;QI Hexing(College of Agriculture and Animal Husbandry,Qinghai University,Xining 810016,China)
出处
《植物医学》
2024年第2期49-57,共9页
Plant Health and Medicine
基金
青海省自然科学基金项目(2022-ZJ-977Q)。
关键词
沙棘
生防内生真菌
产酶活性
纤维素酶
Hippophae rhamnoides L.
biocontrol endophytic fungi
enzyme producing activity
cellulase
