摘要
目的探讨小建中汤治疗胃溃疡的分子机制。方法通过检索中药系统药理学数据库和分析平台(TCMSP),并结合中国知网、万方数据库的相关文献,收集小建中汤各药物包含的主要活性成分;通过TCMSP,PubChem,Swiss TargetPrediction数据库收集小建中汤的潜在作用靶点,通过GeneCards,OMIM,Drugbank,Therapeutic Target Database(TTD)数据库筛选胃溃疡相关的靶点,绘制维恩图,筛选出小建中汤与胃溃疡的交集靶点(关键靶点);再使用Cytoscape 3.7.1软件构建药物-活性成分-疾病-靶点网络,通过String数据库构建关键靶点的蛋白相互作用(PPI)网络;通过Metascape数据库进行基因本体论(GO)功能富集和京都基因与基因组百科全书(KEGG)通路富集分析;预测君药饴糖治疗胃溃疡的关键靶点。结果共收集到方剂潜在活性成分142种及靶点282个,潜在疾病靶点711个,关键靶点111个,度值排名靠前的关键靶点有ALB,AKT1,TP53,IL-6,TNF,IL-1β,JUN,VEGFA。GO功能富集分析得1894种功能,其中生物学过程1685种,分子功能133种,细胞组成76种,主要包括细胞运动的正调控、蛋白激酶结合、胞质囊腔等;KEGG通路富集分析得178条信号通路,主要包括PI3K/Akt信号通路、MAPK信号通路等。君药饴糖主要通过作用于VEGFA,STAT3等靶点促进溃疡愈合。结论小建中汤可通过多成分、多靶点、多通路对胃溃疡发挥疗效。
Objective To investigate the molecular mechanism of Xiaojianzhong Decoction in the treatment of gastric ulcer.Methods Based on the relevant studies in the CNKI and WanFang databases,the main active ingredients in traditional Chinese medicines in Xiaojianzhong Decoction were collected by the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP).The potential targets of Xiaojianzhong Decoction were collected by the TCMSP,PubChem,and Swiss TargetPrediction databases.The gastric ulcer-related targets were screened by the GeneCards,OMIM,Drugbank and Therapeutic Target Database(TTD).The Venn diagram was drawn to screen the common targets(key targets)of Xiaojianzhong Decoction and gastric ulcer.A network of drug-active ingredient-disease-target was constructed by the Cytoscape 3.7.1 software,and a protein-protein interaction(PPI)network for key targets was constructed by the String database.The Gene Ontology(GO)functional enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses were performed by the Metascape database.The key targets of maltose(principal drug)in the treatment of gastric ulcer were predicted.Results A total of 142 potential active ingredients,282 targets in the prescription,711 potential disease targets and 111 key targets were collected.The key targets with high degree included ALB,AKT1,TP53,IL-6,TNF,IL-1β,JUN and VEGFA.The GO functional enrichment analysis showed that 1894 functions were obtained,among which there were 1685 biological processes,133 molecular functions,and 76 cell compositions,mainly including positive regulation of cell motility,protein kinase binding,cytoplasmic vesicle lumen and so on.The KEGG pathway enrichment analysis showed that 178 signaling pathways were obtained,mainly including PI3K/Akt signaling pathway,MAPK signaling pathway and so on.Maltose(principal drug)promoted the ulcer healing mainly through the targets such as VEGFA and STAT3.Conclusion Xiaojianzhong Decoction treats gastric ulcer through multiple ingredients,multiple targets and multiple pathways.
作者
张雪薇
李镇
陈璇
ZHANG Xuewei;LI Zhen;CHEN Xuan(Digestive Endoscopy Treatment Center,The Second Affiliated Hospital of Nanjing University of Traditional Chinese Medicine,Nanjing,Jiangsu,China 210000)
出处
《中国药业》
CAS
2024年第10期70-76,共7页
China Pharmaceuticals
基金
江苏省中医临床优秀人才研修项目[苏中医科教〔2022〕1号]。
关键词
小建中汤
胃溃疡
网络药理学
分子机制
饴糖
Xiaojianzhong Decoction
gastric ulcer
network pharmacology
molecular mechanism
maltose
