摘要
目的探讨扶正化瘀胶囊(FZHY)对肝纤维化发展的影响。方法CCK-8法检测FZHY对LX-2细胞活性的影响。RT-qPCR和Western blot法测定LX-2转染TGF-β或/和给予FZHY后α-SMA水平。选取2022年1月至2022年12月在上海第九人民医院接受抗病毒治疗的慢性乙型肝炎患者80例作为研究对象,分为对照组和治疗组,治疗组每日口服扶正化瘀胶囊1.5 g,一日3次,瞬时弹性成像检查各组治疗前后瞬时肝弹性F值。结果FZHY明显抑制LX-2生长,并与剂量呈正相关(P<0.05)。过表达TGF-β后α-SMA mRNA明显增加(57.14±4.81),与对照组相比差异显著(P<0.001)。同时予10%或20%FZHY时,α-SMA mRNA不同程度降低(37.42±3.65及21.79±0.496),与TGF-β组相比,α-SMA mRNA降低差异有统计学意义(P<0.05,P<0.001)。Western blot显示,FZHY能部分抵消TGF-β引起的α-SMA表达上调。治疗组瞬时肝弹性F值较干预前有所下降,但差异无统计学意义(P>0.05)。结论FZHY可通过影响LX-2细胞增殖、调节α-SMA表达来实现抗纤维化作用。
Objective To investigate the effect of Fuzheng Huayu Capsules(FZHY)on the progression of hepatic fibrosis.Methods The impact of FZHY on the activity of LX-2 cells was evaluated using the CCK-8 assay.The expression levels ofα-SMA in TGF-β-transfected LX-2 cells were assessed using RT-qPCR and Western blot analysis after treatment with or without FZHJ.A total of 80 patients with chronic hepatitis B undergoing antiviral treatment at Shanghai Ninth People's Hospital between January 2022 and December 2022 were enrolled in the study and divided into a control group and a treatment group.The treatment group received 1.5 g of Fuzheng Huayu Capsules three times daily,and liver elasticity F-value were measured using transient elastography before and after treatment in both groups.Results The inhibitory effect of FZHY on LX-2 cells growth was significantly dose-dependen(P<0.05).Overexpression of TGF-βsignificantly inceasedα-SMA mRNA level(57.14±4.81)compared to the control group(P<0.001).Treatment with 10%or 20%FZHY resulted in a reduction inα-SMA mRNA levels(37.42±3.65 and 21.79±0.496)compared to the TGF-βgroup,with significant differences(P<0.05,P<0.001).Western blot analysis demonstrated that FZHY partially reversed the upregulated expression ofα-SMA induced by TGF-β.While the liver elasticity F value decreased in the treatment group after therapy,but there was no statistically significant difference(P>0.05).Conclusion FZHY exhibits antifibrotic effects by influencing LX-2 cell proliferation and modulatingα-SMA expression.
作者
黄欣
孟祥军
吴莘
HUANG Xin;MENG Xiang-jun;WU xin(Department of Gastroenterology,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200011,China;Department of Emergency,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200011,China)
出处
《肝脏》
2024年第4期429-431,452,共4页
Chinese Hepatology
基金
上海市青年科技英才杨帆计划(19YF1426900)。
关键词
肝纤维化
肝星状细胞
扶正化瘀胶囊
Hepatic fibrosis
Hepatic stellate cells
Fuzheng Huayu Capsules
