摘要
目的:探究黑灵芝多糖(PSG-1)对脂多糖(LPS)诱导的IEC-6肠上皮细胞损伤的保护作用及其机制。方法:采用LPS构建肠上皮细胞IEC-6损伤模型,研究PSG-1对IEC-6细胞的干预效果。采用细胞计数盒(cck-8)法测定PSG-1干预对细胞活力的影响。运用western-blot技术探究细胞中肠道紧密连接蛋白和环氧化酶cox-2表达的变化,基于转录组测序技术分析PSG-1潜在的保护机制并对其进行验证。结果:PSG-1干预可以显著提升LPS造成的细胞活力降低和肠道紧密连接蛋白ZO-1、Claudin-1和Occludin的表达,而且PSG-1对LPS引起的cox-2异常高表达具有抑制效果。转录组测序及划痕试验和蛋白免疫印迹试验结果表明:PSG-1能显著增强细胞的迁移能力并抑制促凋亡蛋白Bax、Caspase-3和Caspase-9的表达。结论:PSG-1对LPS诱导的肠上皮细胞IEC-6具有显著的保护作用,细胞迁移和凋亡可能是PSG-1发挥其保护效应的关键途径。
Objective:To investigate the protective effect of Ganoderma atrum polysaccharide(PSG-1)against lipopolysaccharide(LPS)-induced damage in intestinal epithelial cell(IEC-6)and its underlying mechanisms.Methods:The intervention effects of PSG-1 on IEC-6 cells were investigated using a LPS-induced intestinal epithelial cell IEC-6 injury model,and the effect of PSG-1 intervention on cell viability was assessed using the cell counting kit-8(CCK-8)assay.Western blot technology was employed to investigate the alterations in the expression of intestinal tight junction proteins and cyclooxygenase-2(cox-2)in the cells.Transcriptome sequencing analysis was conducted to explore the potential protective mechanisms of PSG-1,followed by experimental validation.Results:Intervention with PSG-1 significantly enhanced cell viability and the expression of intestinal barrier proteins,including ZO-1,Claudin-1,and Occludin.Moreover,PSG-1 exhibited an inhibitory effect on the abnormal upregulation ofcox-2 induced by LPS.Transcriptome sequencing analysis,scratch assay,and western blot experiments validation demonstrated that PSG-1 significantly enhanced cell migration ability while suppressing the expression of pro-apoptotic proteins,including Bax,Caspase-3,and Caspase-9.Conclusion:PSG-1 exhibited significant improvement in LPS-induced damage in IEC-6 cell.Cell migration and apoptosis might be key mechanisms through which PSG-1 exerted its protective effects.
作者
郑冰
胡晓波
陈奕
谢建华
余强
Zheng Bing;Hu Xiaobo;Chen Yi;Xie Jianhua;Yu Qiang(State Key Laboratory of Food Science and Resources,China-Canada Joint Lab of Food Science and Technology(Nanchang),Nanchang University,Nanchang 330047)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2024年第4期43-53,共11页
Journal of Chinese Institute Of Food Science and Technology
基金
国家自然科学基金地区科学基金项目(81760737)。
关键词
黑灵芝多糖
脂多糖
肠上皮细胞IEC-6
细胞迁移
凋亡
Ganoderma atrum polysaccharide
lipopolysaccharide
intestinal epithelial cells(IEC-6)
cell migration
apoptosis
