摘要
为得到高效的柑橘砂皮病生防资源,从环境中分离纯化具有拮抗活性的功能菌,通过与柑橘砂皮病病原菌进行对峙培养,筛选出对砂皮病原菌抑制率达80.14%的菌株QD15。通过形态学、生理生化特征以及分子生物学技术对菌株QD15进行鉴定,确认其为娄彻氏链霉菌(Streptomycete rochei)。QD15除了对柑橘砂皮病菌具有拮抗作用外,对辣椒疫霉、小麦赤霉病菌、烟草根黑腐病菌、水稻稻瘟病菌、香蕉大茎点菌以及黄瓜炭疽病菌等多个病原真菌都有抑菌效果,抑菌率为50.36%~78.66%。QD15抑菌物质60℃及以下处理抑菌率无明显影响,80℃处理60 min抑菌率为48.52%;碱性条件下较为稳定,但在酸性条件下抑菌率明显下降;在紫外线灯照射180 min后抑菌率为70.57%;盆栽试验结果表明,QD15发酵液稀释10倍对柑橘砂皮病防治效果为67.22%。试验结果表明,娄彻氏链霉菌QD15可进一步研发为柑橘砂皮病等作物病害生物防控产品。
To obtain highly efficient biocontrol agents targeting citrus melanose,the functional bacteria with antagonistic activities were isolated from the surroundings and purified.Through confrontation culture with the pathogenic fungus(Diaporthe citri)of citrus melanose,strain QD15 with an inhibition rate against D.citri up to 80.14%was screened out,which was identified as Streptomycete rochei based on the morphological,physiological,biochemical characteristics and molecular biotechnological analysis.In addition to its resistance to D.citri,QD15 also exhibited antifungal effect against multiple pathogenic fungi such as Phytophthora capsici,Fusarium asiaticum,Thielaviopsis basicola,Magnaporthe oryzae,Macrophoma musae,and Colletotrichum orbiculare,with the inhibition rate ranging from 50.36%to 78.66%.The antifungal efficacy of QD15 did not change significantly under the treatment at 60°C and lower temperatures,but decreased to 48.52%under the treatment at 80°C for 60 min.Moreover,its antifungal activity was stable in alkaline conditions whereas decreased notably in acidic conditions.After ultraviolet radiation for 180 min,the inhibition rate was 70.57%.Pot experiment results revealed that the fermentation liquor of QD15,diluted tenfold,achieved a substantial control effect against citrus melanose,with an efficacy rate of 67.22%.In conclusion,QD15 presents promising potential for further investigation and development as a biocontrol agent against citrus melanose and other crop diseases.
作者
谢钢
裴皓然
张家辉
刘倩
姚翔
陈添煜
戴良英
王运生
XIE Gang;PEI Hao-ran;ZHANG Jia-hui;LIU Qian;YAO Xiang;CHEN Tian-yu;DAI Liang-ying;WANG Yun-sheng(College of Plant Protection,Hunan Agricultural University,Changsha 410128,PRC)
出处
《湖南农业科学》
2024年第4期60-66,共7页
Hunan Agricultural Sciences
基金
国家重点研发计划(2021YFD1400805)。
关键词
柑橘砂皮病
娄彻氏链霉菌
抑菌率
稳定性
盆栽试验
citrus melanose
Streptomycete rochei
antifungal rate
stability
pot experiment
