摘要
【目的】厘清天然广布树种栓皮栎叶片解剖结构的种群变异及其与环境因子的关系。【方法】以中国28个天然分布栓皮栎种群叶片为研究对象,用常规石蜡切片结合光学显微技术对栓皮栎叶片解剖性状进行研究,用巢式方差分析、Pearson相关性分析等方法对栓皮栎叶片解剖结构的种群变异与环境因子关系进行分析。【结果】(1)栓皮栎叶片解剖性状在28个种群间存在显著差异,平均变异系数为7.84%~15.16%,且同一叶片解剖性状在不同种群间的变异范围不同;(2)9个解剖性状在种群内及种群间均存在极显著差异,平均表型分化系数为37.44%,解剖性状变异主要来自种群内;(3)叶片厚度、上表皮厚度、栅栏组织厚度、海绵组织厚度与纬度呈显著正相关关系,年均气温及年降水量对上表皮厚度和栅栏组织厚度有显著影响。【结论】栓皮栎具有丰富的遗传多样性,为适应低温与干旱,栓皮栎叶片整体呈增厚趋势。研究结果为了解栓皮栎环境适应策略提供理论依据。
[Objective]The study aims to understand the population variation of leaf anatomical structure of Quercus variabilis and its relationship with environmental factors.[Methods]The leaves of 28 naturally distributed Q.variabilis populations in China were studied by conventional paraffin sections combined with optical microscopy.Nested analysis of variance and Pearson correlation analysis were performed to analyze the relationship between population variation of leaf anatomical structure and environmental factors.[Results](1)There were significant difference in the anatomic traits of Q.variabilis leaves among 28 populations,and the average coefficient of variation was 7.84%-15.16%,while the variation range of the same leaf anatomic trait varied among different populations.(2)There were significant difference among the 9 anatomic traits within and among the populations,and the average phenotypic differentiation coefficient was 37.44%.The variation of anatomic traits mainly came from within the populations.(3)Leaf thickness,upper epidermal cell thickness,palisade tissue thickness,and spongy tissue thickness were significantly positively correlated with latitude,and mean annual temperature and annual precipitation had significant effects on the upper epidermal cell thickness and palisade tissue thickness.[Conclusion]The results show that Q.variabilis has rich genetic diversity.To adapt to low temperature and drought,Q.variabilis leaves show a trend of thickening.The results provide a theoretical basis for understanding environmental adaptation strategies of Q.variabilis.
作者
陈恺茜
郝凡
袁衍琪
刘欣蕊
王雪
张硕新
CHEN Kaixi;HAO Fan;YUAN Yanqi;LIU Xinrui;WANG Xue;ZHANG Shuoxin(College of Forestry,Northwest A&F University,Yangling,Shaanxi 712100,China;Qinling National Forest Ecosystem Research Station,Ningshan,Shaanxi 711600,China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2024年第5期772-781,共10页
Acta Botanica Boreali-Occidentalia Sinica
基金
秦岭太白山不同海拔梯度植物群落功能性状研究项目(2016YFC0500202)。
关键词
栓皮栎
叶片解剖结构
环境因子
相关性
Quercus variabilis
leaf anatomical structure
environmental factors
correlation
