摘要
【目的】探究高浓度氯盐胁迫下桧柏容器苗施加不同质量分数黄腐酸钾(FA-K)后的生长和生理特性,揭示FA-K缓解高浓度氯盐胁迫下桧柏的生理响应机制及差异性,为冬季北方高速公路中央分隔绿化带桧柏的管理维护提供技术支撑。【方法】选取2年生桧柏容器苗开展盆栽试验,以正常生长为对照,设置0.7%NaCl和0.7%CaCl_(2)2种盐分,每种盐分设置0、0.05%、0.10%、0.30%和0.50%5种质量分数FA-K处理,测定各处理下桧柏苗生长指标(苗高生长量、地径生长量、生物量、根冠比)、光合色素含量(叶绿素a、叶绿素b、总叶绿素、类胡萝卜素)、相对电导率、丙二醛含量、渗透调节物质(脯氨酸、可溶性糖)含量、超氧化物歧化酶和过氧化物酶活性。【结果】1)0.7%CaCl_(2)胁迫对桧柏苗生长的抑制作用大于0.7%NaCl胁迫;0.7%CaCl_(2)胁迫下桧柏苗除可溶性糖和类胡萝卜素含量低于NaCl胁迫外,其他生理指标(叶绿素a含量、叶绿素b含量、总叶绿素含量、丙二醛含量、脯氨酸含量、相对电导率、过氧化物酶和超氧化物歧化酶活性)均高于NaCl胁迫;2)2种盐胁迫下,施加FA-K均使桧柏苗苗高生长量、地径生长量、生物量增加,叶绿素a、叶绿素b、总叶绿素、类胡萝卜素含量上升,相对电导率和丙二醛含量下降,超氧化物歧化酶和过氧化物酶活性、脯氨酸和可溶性糖含量呈低-高-低的变化趋势,0.50%FA-K下2种盐胁迫的幼苗生长接近对照;3)相较0.7%NaCl胁迫,FA-K对0.7%CaCl_(2)胁迫表现出更好的缓解作用。【结论】高浓度氯盐胁迫显著抑制桧柏苗生长和存活,0.7%CaCl_(2)抑制作用比0.7%NaCl强;FA-K能够有效缓解NaCl和CaCl_(2)胁迫,0.50%FA-K缓解效果较佳。
【Objective】In this paper,we studied the growth and physiological characteristics of Sabina chinensis vulgaris container seedlings under high concentration salt stress,and the mitigation effect and difference of fulvic acid potassium on high concentration NaCl and CaCl_(2) stress.It provides technical support for the management and maintenance of median strip of northern expressway in winter.【Method】We used 2-year-old Sabina chinensis container seedlings as study materials during pot experiment,and normal growth as control.The effects of potassium fulvic acid(FA-K)at 0,0.05%,0.10%,0.30%and 0.50%on alleviating high salt stress of 0.7%NaCl and CaCl_(2) were investigated.The growth indexes of Sabina chinensis seedlings(seedling height growth,ground diameter growth,biomass,root-shoot ratio),photosynthetic pigment content(chlorophyll a,chlorophyll b,total chlorophyll,carotenoid),relative conductivity,MDA content,osmotic adjustment substance content(proline,soluble sugar),superoxide dismutase(SOD)and peroxidase(POD)were measured under various treatments.【Result】1)The inhibition effect of 0.7%CaCl_(2) stress on the growth of Sabina chinensis seedlings was greater than that of 0.7%NaCl stress.Under 0.7%CaCl_(2) stress,other physiological indexes(chlorophyll a,chlorophyll b,total chlorophyll,MDA,proline content,relative conductivity,POD,SOD activity)were higher than those under NaCl stress except that the content of soluble sugar and carotenoid was lower than that under NaCl stress.2)Under two kinds of salt stress,fulvic acid potassium increased height growth,ground diameter growth and biomass,increased chlorophyll a,chlorophyll b,total chlorophyll and carotenoid content,decreased relative electrical conductivity and MDA content,and showed a low-high-low trend in SOD and POD activity,prominence acid and soluble sugar content.Under 0.50%FA-K,the seedling growth under the two salt stresses was close to the control.3)Compared with 0.7%NaCl stress,fulvic acid potassium showed better mitigation effect on 0.7%CaCl_(2) stress.【Conclusion】The result indicated that high salt significantly inhibited the growth and survival of Sabina chinensis,and the inhibition effect of 0.7%CaCl_(2) was stronger than that of 0.7%NaCl.The stress of NaCl and CaCl_(2) could be effectively mitigated by fulvic acid potassium,and 0.50%of fulvic acid potassium was most effective.
作者
房有鑫
曹帮华
郭龙梅
毛培利
庞元湘
张金颢
王志恒
李鹏飞
Fang Youxin;Cao Banghua;Guo Longmei;Mao Peili;Pang Yuanxiang;Zhang Jinhao;Wang Zhiheng;Li Pengfei(Key Laboratory of National Forestry and Grassland Administration for the Cultivation of Forests in the Lower Reaches of the Yellow River Shandong Agricultural University,Tai’an 271018)
出处
《林业科学》
EI
CAS
CSCD
北大核心
2024年第4期99-108,共10页
Scientia Silvae Sinicae
基金
山东高速集团项目“G35济广高速华山立交北匝道圈绿化提升工程”(JQJNYG(S)-2022-006)。
关键词
桧柏
盐胁迫
黄腐酸钾
融雪剂
缓解作用
Sabina chinensis
salt stress
fulvic acid potassium
deicing salt
physiological characteristics
