摘要
目的:观察“脑心同治”电针法的不同介入时间对创伤性脑损伤(TBI)后认知障碍的影响。方法:将80只雄性SD大鼠随机分成假手术组、模型组、电针1组、电针2组和电针3组,每组均为16只。除假手术组外,其余大鼠均制备TBI模型。电针1组、电针2组和电针3组分别在造模后即刻、造模后第3天、造模后第7天开始针刺,治疗持续14 d。观察各组大鼠mNSS神经功能评分、新物体识别实验和Morris水迷宫实验的表现;随后采用HE以及FJB染色法观察各组大鼠损伤灶周围及患侧海马区组织形态变化;Western blot(WB)及免疫荧光检测各组大鼠损伤灶周围IL-1β、IL-18的蛋白表达及分布。结果:与模型组比较,所有电针组均能显著降低mNSS评分,提高大鼠对新物体探索的时间与偏好指数,缩短大鼠逃避潜伏期、提高穿越平台次数,并降低IL-1β、IL-18的表达与分布,差异具有统计学意义(P<0.05)。且FJB染色结果显示电针组大鼠大脑中发生退行性变异的神经元细胞数量明显少于模型组,差异具有统计学意义(P<0.05),且电针1组与假手术组比较差异无统计学意义(P>0.05),HE染色结果显示电针组大鼠患侧海马区组织形态较为完整。其中电针1组各项指标结果均优于电针2组和电针3组,在新物体识别实验、水迷宫实验、WB与免疫荧光检测中,假手术组与电针1组各项指标比较差异无统计学意义(P>0.05)。结论:“脑心同治”电针法具有改善TBI大鼠认知功能的作用,且介入时间越早,伤后即刻电针抑制神经炎症、减轻神经元损伤和改善TBI后认知障碍的作用越显著。
Objective:To observe the effects of different electro-acupuncture(EA) intervention time on cognitive impairment after traumatic brain injury(TBI) based on ‘Brain-Heart concurrent regulation'.Methods:80 male SD rats were randomly divided into the sham operation group,the model group,the EA group 1,the EA group 2,and the EA group 3,with 16 rats in each group.Except for those in the sham operation group,the rats in the rest groups were prepared for the TBI model.EA was administered immediately after modeling in the EA group 1,on the 3 rd day after modeling in the EA group 2 and on the 7 th day after modeling in the EA group 3,and the EA intervention duration was 14 days.The scores of mNSS neurological function,and the performances in novel object recognition test and Morris water maze test were observed.HE staining and FJB staining were used to observe the morphological changes in the tissues around the injury site and the hippocampal region of the affected side.Western blot(WB) and immunofluorescence were used to detect the protein expressions and distributions of IL-1β and IL-18 in the peri-lesion and hippocampus.Results:Compared with those in the model group,the mNSS scores were decreased,the exploration time and preference index for novel objects were increased,the escape latency was shortened,the number of platform crossings in the Morris Water Maze Test was increased,and the expressions and distributions of IL-1β and IL-18 were decreased in the EA groups(P<0.05).The FJB staining results showed that the number of degenerated neurons in the brains was significantly lower in the EA groups than that in the model group(P<0.05),and in which there was no statistical difference between the EA group 1 and the sham operation group(P>0.05).HE staining results showed a more intact morphology of the affected hippocampal region in the EA groups.Among the above indicators,the results in the EA group 1 were better than those in the EA group 2 and the EA group 3.In terms of the indicators in novel object recognition test,Morris water maze test,WB and immunofluorescence detection,there was no statistical differences between the sham operation group and the EA group 1(P>0.05).Conclusion:The ‘Brain-Heart concurrent regulation' EA therapy has the effect of improving cognitive function in TBI rats.The earlier the intervention,i.e.,acupuncture immediately after injury,the more significant effect can be achieved in suppressing neuroinflammation,reducing neuronal damage,and improving cognitive impairment after TBI.
作者
郭文慧
张卫
郑淑美
苑鸿雯
张璐
王天琪
崔学慧
崔海
杜若桑
GUO Wenhui;ZHANG Wei;ZHENG Shumei;YUAN Hongwen;ZHANG Lu;WANG Tianqi;CUI Xuehui;CUI Hai;DU Ruosang(Capital Medical University,Beijing 100069,China;Xuanwu Hospital of Traditional Chinese Medicine,Beijing 100050,China)
出处
《针灸临床杂志》
2024年第4期82-90,共9页
Journal of Clinical Acupuncture and Moxibustion
基金
北京市教育委员会科研计划,编号:KM202310025018
首都医科大学校培育基金(2022年)。
关键词
创伤性脑损伤
认知障碍
脑心同治
电针治疗
介入时间
Traumatic brain injury
Cognitive impairment
Brain-Heart concurrent regulation
Electro-acupuncture treatment
Intervention time
