摘要
为研究植物工厂环境下不同光强对陆地棉(Gossypium hirsutum L.)生长发育的影响,本试验在植物工厂内,以陆地棉品种湘FZ001为试验品种,采用16 h光照、8 h暗期循环,设置3种光强处理(L1为450μmol∙m^(-2)∙s^(-1),L2为600μmol∙m^(-2)∙s^(-1),L3为750μmol∙m^(-2)∙s^(-1)),测量棉花的各器官干重,使用直尺测量棉花株高,用SPAD-502叶绿素仪测量棉花叶片的叶绿素相对含量,使用Flour Pen 110测量棉花的初始荧光(F_(0))、最大荧光(F_(m))等6个叶绿素荧光参数。L1光强处理下棉花的叶绿素相对含量和株高最大,L2光强处理下棉花的根干重最大,3种光强处理下的棉花茎、叶和单株总重从高到低依次为L3>L2>L1,F_(0)和F_(m)从高到低依次为L1>L2>L3,最大光化学量子产量(F_(v)/F_(m))从高到低依次为L2>L3>L1。同一光强处理下棉花的光化学猝灭系数(qp)、有效量子产量(φPSII)变化曲线相同,3种光强处理下棉花的qp、非光化学猝灭(NPQ)、φPSII在生长前期差异不大,后期差异较大。从生长后期来看,3种光强处理下棉花的qp、φPSII从高到低依次为L3>L2>L1,NPQ从高到低依次为L2>L3>L1。棉花单株总干重、茎干重、叶干重在高光强下较大,光强太高或太低都会抑制棉花根的生长,低光强可促进棉花的株高。与L1和L3光强处理下的棉花相比,L2光强适宜棉花的生长,既避免了棉花植株间争光,也避免了光抑制现象,叶绿素含量适中,使得棉花的光化学效率最高。本研究可为棉花工厂化生产和棉花育种加速器研发与应用提供指导。
Xiang FZ001 was used as a test variety in this experiment,which was conducted in a plant factory with a cycle of 16 hours of light and 8 hours of darkness to study the effects of varying light intensities on the growth and development of cotton(Gossypium hirsutum L.),three light intensity treatments with parameters of L1:450μmol∙m^(-2)∙s^(-1),L2:600μmol∙m^(-2)∙s^(-1),and L3:750μmol∙m^(-2)∙s^(-1),were established to determine the dry weight of each cotton organ.The height of the cotton plant was measured using a straightedge,the chlorophyll content of cotton leaves was measured using a SPAD-502 chlorophyll meter.Additionally,six chlorophyll fluorescence parameters were measured,including initial fluorescence(F_(0))and maximum fluorescence(F_(m)),using a Flour Pen 110.The relative chlorophyll content and plant height of cotton were greatest under the L1 light intensity treatment,the root dry weight of cotton was greatest under the L2 light intensity treatment,and the total weight of cotton stems,leaves,and individual plants under the three light intensity treatments were L3>L2>L1 in descending order,and the F_(0) and F_(m) were L1>L2>L3 in descending order.The maximum photochemical quantum yield(F_(v)/F_(m))was L2>L3>L1 in descending order.The photochemical bursting coefficient(qp)and effective quantum yield(φPSII)of cotton under the same light intensity treatment showed the same curves of change.During the pre-growth era,there was no significant difference in the qp,φPSII,and nonphotochemical burst(NPQ)of cotton under the three light intensity treatments.However,in the late period,there was a greater difference.For the late growth stage,cotton’s qp andφPSII under the three light intensity treatments were high to low in the order of L3>L2>L1,and high to low in the order of L2>L3>L1.High light intensities increase the dry weight,stem dry weight,and leaf dry weight of cotton plants;low light intensities enhance the height of the cotton plant,but too high or too low a light intensity prevents the growth of cotton roots.Compared with cotton under L1 and L3 light intensity treatments,L2 light intensity was suitable for cotton growth,avoiding both inter-plant competition for light and the phenomenon of photoinhibition,with moderate chlorophyll content,resulting in the highest photochemical efficiency of cotton.This study can provide a guidance for cotton factory production and cotton breeding accelerator development and application.
作者
卢建祥
高倩文
高志强
陈浩东
阳会兵
LU Jianxiang;GAO Qianwen;GAO Zhiqiang;CHEN Haodong;YANG Huibing(College of Agriculture,Hunan Agricultural University,Changsha 410128,China;Institute of Cotton Sciences of Hunan Province,Changde 415101,China)
出处
《激光生物学报》
CAS
2024年第2期115-122,共8页
Acta Laser Biology Sinica
基金
2023年湖南省棉花科技创新专项项目(湘财建指【2023】98号)
2022年湖南省棉花科技创新专项项目(湘财建指【2022】51号)
2021年湖南省自然科学基金项目(2021JJ30348)。
关键词
植物工厂
光强
陆地棉
生长发育
光合特性
plant factory
light flux density
Gossypium hirsutum L.
growth and development
photosynthetic characteristics
