摘要
目的为了降低肿瘤切除术过程中小鼠的围手术期疼痛,保障动物福利最大化和研究数据的准确性,本研究探究美洛昔康对荷瘤小鼠的围手术期镇痛作用。方法所有BALB/c小鼠均采用舒泰和右美托嘧啶的麻醉方案,分为对照组(A组)和美洛昔康注射组(B组)。采用面部表情评分系统和疼痛行为频率评估小鼠的疼痛程度;监测小鼠的体质量以及脏器的组织病理学方法评估其围手术期安全性。结果B组小鼠的麻醉诱导时间较A组缩短,苏醒时间较A组显著延长(P<0.01);术后3 d内,B组小鼠的面部表情评分和疼痛行为频率较A组显著降低;两组小鼠的主要脏器和肠道组织在组织病理学上均无明显病变,体质量无显著差异,B组小鼠全部存活。结论舒泰和右美托嘧啶麻醉方案联合美洛昔康可以缩短BALB/c小鼠的麻醉诱导时间并使其缓慢苏醒,显著地降低了小鼠的围手术期疼痛,且是安全的。
Objective To reduce perioperative pain in mice during tumor resection,ensure animal welfare and the accuracy of research data,the perioperative analgesic effect of meloxicam on tumor-bearing mice were explored in this study.Method All BALB/c mice were anesthetized with Zoletil and Dexmedetomidine,and then divided into the control group(group A)and meloxicam injection group(group B).The mouse grimace scale and pain behavior frequency were evaluated to assess the pain level of mice,and the body weight and the histopathology of the organs were observed to test the safety of meloxicam.Result The result showed that compared with group A,the anesthesia induction time and the recovery time in group B were shorter,while the recovery time in group B was significantly longer(P<0.01);the mouse grimace scale and pain behavior frequency in group B were significantly lower than group A within the first 3 days;the main organs and intestinal tissues of all mice had no histopathological lesions,the weight of mice showed no significant difference,and all the mice in group B were survived.Conclusion The result suggested that meloxicam combined Zoletil and Dexmedetomidine anesthesia regimen could shorten the induction of anesthesia and slowly lengthen the recovery time of mice bearing with tumors,significantly reduce their perioperative pain,and be safe.
作者
刘师佳
高硕磊
覃尧
张红
LIU Shijia;GAO Shuolei;QIN Yao;ZHANG Hong(College of Animal Science and Technology,Hainan University,Haikou 570228,China)
出处
《实验动物科学》
2024年第2期28-34,共7页
Laboratory Animal Science
基金
国家自然科学基金(32260904)。
