摘要
目的探究电针“足三里”(ST36)与“太冲”(LR3)对功能性消化不良(FD)模型大鼠胃肠道动力障碍、胃肠激素及脑-肠轴的影响。方法48只SD大鼠按随机数字表法分为正常对照组、模型组、西沙比利组及电针组,每组12只。采用轻度夹尾刺激配合间断饮食法构建FD大鼠模型。记录各组大鼠造模前后体质量、进食量和糖水偏好率,验证FD模型是否复制成功。造模成功后,正常对照组和模型组不做任何干预;西沙比利组大鼠灌胃西沙比利[0.4 g/(kg·d)],连续6 d为1个疗程,隔日继续下一疗程;电针组大鼠接受双侧“足三里”“太冲”电针刺激(直流电,疏密波,2/100 Hz,2~5 mA。通电30 min),每隔24 h刺激1次,连续6次为1个疗程,隔日继续下一疗程。2个疗程后取材,比较各组大鼠胃肠动力指标,血液胃肠激素[胃动素、胃泌素、5-羟色胺(5-HT)、一氧化氮(NO)]水平,血清、下丘脑、胃窦、十二指肠内源性脑肠肽[促胆囊收缩素(CCK)、降钙素相关基因肽(CGRP)、神经肽Y(NPY)]水平,下丘脑、脊髓、胃窦、结肠组织中胃饥饿素和P物质的蛋白表达。结果模型大鼠体质量、进食量和糖水偏好率均较正常大鼠降低(P<0.05)。与模型组比较,电针组与西沙比利组大鼠胃内残留率降低,小肠推进率、胃电慢波主频率和主功率升高(P<0.05);胃动素、胃泌素、5-HT水平升高,NO水平降低(P<0.05);血清CCK含量增加、CGRP和NPY含量降低(P<0.05);下丘脑、胃窦、十二指肠CCK蛋白阳性表达细胞增加,CGRP和NPY蛋白阳性表达细胞减少(P<0.05);下丘脑、脊髓、胃窦、结肠组织中胃饥饿素蛋白表达上调,P物质蛋白表达下调(P<0.05)。西沙比利组和电针组比较,各指标差异均无统计学意义。结论电针“足三里”与“太冲”可有效缓解FD大鼠胃肠道动力障碍,调节胃肠激素水平,纠正异常脑肠肽表达,调节脑-肠互动平衡。
Objective We aimed to investigate the effects of electroacupuncture at"Zusanli"(ST36)and"Taichong"(LR3)on gastrointestinal motility,gastrointestinal hormones,and brain-gut axis in rats with functional dyspepsia(FD).Methods 48 SD rats were randomly divided into a normal control group,a model group,a cisapride group,and an electroacupuncture group,with 12 rats in each group.A mild tail pinch stimulation combined with intermittent food deprivation was used to establish the FD rat model.Body mass,food intake,and sucrose preference rate were recorded before and after modeling to verify the success of the FD model.After modeling,the normal control group and the model group received no intervention;the cisapride group rats were administered cisapride[0.4 g/(kg·d)]by intragastric perfusion for 6 consecutive days as one course of treatment,with a rest day between each course;the electroacupuncture group rats received electroacupuncture stimulation at bilateral"Zusanli"and"Taichong"(DC current,dense-sparse wave,2/100 Hz,2-5 mA.Electricity was applied for 30 min),with stimulation every 24 h for 6 consecutive times as one course of treatment,with a rest day between each course.After two courses of treatment,samples were collected to compare gastrointestinal motility indices,blood levels of gastrointestinal hormones[motilin,gastrin,serotonin(5-HT),nitric oxide(NO)],serum and tissue levels of endogenous brain-gut peptides[cholecystokinin(CCK),calcitonin gene-related peptide(CGRP),neuropeptide Y(NPY)]in the hypothalamus,stomach,duodenum,colon,and protein expression of ghrelin and substance P in the hypothalamus,spinal cord,stomach,and colon.Results Body mass,food intake,and sucrose preference rate were lower in the model rats than in the normal rats(P<0.05).Compared with the model group,rats in the electroacupuncture group and cisapride group had(ⅰ)lower gastric residual rates,higher rates of small intestinal propulsion and gastric slow wave main frequency and power(P<0.05);(ⅱ)levels of motilin,gastrin,and 5-HT increased,while NO levels decreased(P<0.05);(ⅲ)serum CCK content increased,while CGRP and NPY levels decreased(P<0.05);(ⅳ)CCK protein positive expression increased,while CGRP and NPY protein positive expression decreased in the hypothalamus,stomach,duodenum(P<0.05);(ⅴ)ghrelin protein expression increased,and substance P protein expression decreased in the hypothalamus,spinal cord,stomach,and colon(P<0.05).There were no significant differences in these indicators between the electroacupuncture group and the cisapride group.Conclusion Electroacupuncture at"Zusanli"and"Taichong"can effectively alleviate gastrointestinal motility disorders in FD rats,regulate gastrointestinal hormone levels,reverse abnormal brain-gut peptide expression,and regulate brain-gut interaction balance.
作者
王琼
张艺
邹燃
周利
杨敏
徐派的
WANG Qiong;ZHANG Yi;ZOU Ran;ZHOU Li;YANG Min;XU Paidi(Department of Acupuncture and Moxibustion,First Hospital of Wuhan&Wuhan Hospital of Traditional Chinese and Western Medicine,Wuhan 430030,China;College of Acupuncture and Orthopedics,Hubei University of Chinese Medicine,Wuhan 430065,China)
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2024年第4期570-579,共10页
Journal of Beijing University of Traditional Chinese Medicine
基金
国家自然科学基金青年科学基金项目(No.81704178)
湖北省卫生健康委员会联合基金项目(No.WJ2019H418)
武汉市卫生科研基金项目(No.WZ15A07)。
关键词
电针
足三里
太冲
功能性消化不良
胃肠道动力障碍
脑肠肽
胃肠激素
大鼠
electroacupuncture
Zusanli
Taichong
functional dyspepsia
gastrointestinal motility disorder
brain-gut peptides
gastrointestinal hormones
rats
