摘要
为探讨STAR基因在发育的绵羊睾丸和附睾中的表达模式及潜在功能,该研究采集3月龄、 1周岁和3周岁藏绵羊睾丸和附睾(头、体和尾)组织样本,利用实时荧光定量PCR、蛋白免疫印迹和免疫组织荧光染色法检测STAR基因的表达及其编码蛋白的细胞定位。结果发现,随着年龄的增加,在藏绵羊睾丸和附睾(附睾头除外)中STAR基因在转录水平的表达总体呈下降趋势,而在蛋白质水平的表达呈上调趋势。STAR蛋白主要存在于藏绵羊睾丸间质细胞和附睾假复层纤毛上皮主细胞。鉴于间质细胞和主细胞的主要生物学功能是分泌睾酮和分泌促进精子成熟所需的蛋白质,由此推测,STAR基因可能主要在睾丸间质细胞和附睾主细胞的发育和功能维持中发挥作用,进而促进雄激素的合成和精子的成熟。
To investigate the expression pattern and potential functions of the STAR gene in the developmental sheep testis and epididymis,the testicular and epididymal(caput,corpus and cauda)tissue samples were collected from Tibetan sheep at 3 months,1 year,and 3 years of age.Real-time quantitative PCR,Western blot,and immunofluorescence staining were employed to detect the expression of the STAR gene and cellular localization of its encoded protein.The results indicated that the expression of the STAR gene in testes and epididymis(except for caput)of Tibetan sheep exhibited an overall decrease trend at the transcriptional level,while the expression at the protein level showed an upregulated trend with increasing age.STAR protein was mainly existed in the Leydig cells from testes and the principal cells from epididymal pseudostratified ciliated epithelium.Given that the main biological functions of Leydig cells and principal cells are secretion of testosterone and secretion of proteins required to promote sperm maturation,respectively,it is thus speculated that the STAR gene may mainly play a role in the development and functional maintenance of Leydig cells and epididymal principal cells,thereby promoting androgen synthesis and sperm maturation.
作者
蒋雨婷
王霞
宋娟娟
李晨硕
王彩丽
陈浩林
李讨讨
马友记
Jiang Yuting;Wang Xia;Song Juanjuan;Li Chenshuo;Wang Caili;Chen Haolin;Li Taotao;Ma Youji(College of Animal Science and Technology,Gansu Agricultural University,Lanzhou,730070,Gansu;Gansu Key Laboratory of Animal Generational Physiology and Reproductive Regulation,Lanzhou,730070,Gansu;Guizhou Institute of Animal Husbandry and Veterinary Medicine,Guiyang,550005,Guizhou)
出处
《中国畜禽种业》
2024年第4期77-84,1,共9页
The Chinese Livestock and Poultry Breeding
基金
甘肃省大学生创新创业训练计划项目(S202310733018)
甘肃省自然科学基金青年科技基金(22JR5RA886)
贵州省科技计划项目(黔科合中引地[2023] 018)
国家自然科学基金(31960662)
甘肃农业大学学科团队项目(GAU-XKTD-2022-20)。
