摘要
目的:基于蛋白质组学技术研究通关藤注射液对小鼠肺癌组织生长情况及蛋白表达的影响,探究其作用机制。方法:40只BALB/c雄性小鼠,采用腋下接种Lewis肺癌瘤株的方法建立肺癌皮下移植瘤模型,随机分为模型组、顺铂组和通关藤注射液高、低剂量组,每组10只。分别在给药前及给药后记录小鼠肿瘤生长情况,在给药第21天时处死,眼球取血,取肿瘤组织,称量体积及瘤体质量。通过检测各组小鼠肿瘤大小,计算抑瘤率来评价肿瘤生长情况。取血清样本,采用定量蛋白质组学分析血清中蛋白表达差异的影响,并分析差异蛋白参与的信号通路。结果:与模型组相比,通关藤注射液高剂量和低剂量组肿瘤组织显著减小(P<0.01)。共定量得到6272个蛋白质,给药组与模型组之间有176个差异蛋白(上调59个,下调117个),这些差异蛋白的功能主要为基因表达、细胞生物合成、DNA模板转录等;对差异蛋白富集的信号通路进行分析可知,PI3K/Akt信号通路、HIF-1信号通路、ErbB信号通路、心肌收缩、志贺菌病、系统性红斑狼疮、胰岛素信号通路、不同环境中的微生物代谢、cAMP信号通路、信使核糖核酸监测通路等通路可能与肿瘤细胞的增殖、存活有密切关系。结论:通关藤注射液抑制肺癌小鼠肿瘤生长的作用机制,可能通过调节关键蛋白影响基因表达、细胞生物合成、DNA模板转录等过程,与PI3K/Akt信号通路、HIF-1信号通路、ErbB信号通路等通路密切相关。
Objective:To study the effect of Tongguanteng Injection on the growth and protein expression of lung cancer tissue in mice based on proteomics technology,and explore its mechanism.Methods:Forty BALB/c male mice were selected and a subcutaneous lung cancer transplantation model was established by inoculating Lewis lung cancer tumor strain under the armpit.They were randomly divided into a model group,a cisplatin group,and highdose and low-dose groups of Tongguanteng Injection,with 10 mice in each group.The tumor growth of mice was recorded before and after administration.On the 21st day of administration,the mice were euthanized,blood was collected from the eyeballs,tumor tissue was taken,and the volume and mass of the tumor were measured.Tumor growth was evaluated by measuring tumor size and calculating tumor inhibition rate.Serum samples were taken,and quantitative proteomics was used to analyze the influence of protein expression differences in serum,and the signal pathways involved in the differential proteins were analyzed.Results:Compared with the model group,the tumor tissue in the high-dose and low-dose groups of Tongguanteng Injection was significantly reduced(P<0.01).A total of 6272 proteins were quantitatively obtained,and 176 different proteins(59 up-regulated and 117 downregulated)were detected between the administration group and the model group.The functions of these differential proteins mainly included gene expression,cellular biosynthesis,DNA template transcription,etc.Analysis of signal pathways enriched with differential proteins revealed that the PI3K/Akt signaling pathway,HIF-1 signaling pathway,ErbB signaling pathway,myocardial contraction,shigellosis,systemic lupus erythematosus,insulin signaling pathway,microbial metabolism in different environments,cAMP signaling pathway,messenger RNA monitoring pathway,etc.might be closely related to the proliferation and survival of tumor cells.Conclusion:The mechanism of the inhibitory effect of Tongguanteng Injection on tumor growth in lung cancer mice may be closely related to the PI3K/Akt signaling pathway,HIF-1 signaling pathway,ErbB signaling pathway,and other pathways by regulating key proteins that affect gene expression,cell biosynthesis,DNA template transcription,etc.
作者
贾文瑞
边猛
贾羲
张晓川
王娟
JIA Wenrui;BIAN Meng;JIA Xi;ZHANG Xiaochuan;WANG Juan(The First Affiliated Hospital of Zhengzhou University,Zhenzhou 450000,China)
出处
《中医药信息》
2024年第5期24-30,共7页
Information on Traditional Chinese Medicine
基金
郑州大学第一附属医院横向课题(K2020-0294)。
关键词
通关藤注射液
蛋白质组学
肺癌
作用机制
信号通路
Tongguanteng Injection
Proteomics
Lung cancer
Mechanism of action
Signaling pathway
