摘要
为了探究生长年限对川党参根际土壤细菌群落结构特征及其多样性的影响,阐明细菌群落变化规律,采用Illumina MiSeq高通量测序技术对1年生、2年生、3年生和4年生的川党参根际土壤细菌16S rRNA序列进行测序分析。结果表明:(1)生长年限显著影响川党参根际土壤细菌群落多样性,其OTU数、Alpha多样性指数均随生长年限增加呈先升高后降低的趋势;(2)生长年限改变了细菌群落组成,但在门水平上优势菌门(相对丰度>5%)相对稳定,均为变形菌门(Proteobacteria)、未分类细菌门(unclassified_Bacteria)、酸杆菌门(Aci-dobacteria)、放线菌门(Actinobacteria)、拟杆菌门(Bacteroidetes)和绿弯菌门(Chloroflexi),其相对丰度总和占85.71%~91.39%;在属水平上优势菌群相差较大,仅有全噬菌属(Holophaga)和念珠菌属(Candidatus Udaeo-bacter)是4个年限所共有的优势菌属,革兰氏阴性菌cTPY-13(Gram-negative bacterium cTPY-13)则为2年生独有优势菌属,其余则分别为不同年限有相应共有优势菌属;(3)PCoA分析与UPGMA聚类分析显示,各生长年限间细菌群落差异明显,随生长年限增加,不同组间细菌群落结构差异先增大后逐渐减小,细菌群落在2~3年生时演变较为剧烈;(4)土壤速效养分是影响根际土壤细菌群落的主要环境因子,其程度大小为水解氮(HN)>有效磷(AP)>速效钾(AK),优势菌门中酸杆菌门与AP、AK呈显著负相关(P<0.05),拟杆菌门与AP、AK呈显著正相关(P<0.05),而绿弯菌门与HN呈显著负相关(P<0.05)。综上所述,川党参生长年限延长会影响根际土壤细菌群落结构和多样性,从而改变其根际微生态系统。
In order to explore the effects of growth years on the characteristics and diversity of bacterial community structure in the rhizosphere soil of Codonopsis tangshen and explain the change rule of bacterial community,Illumina MiSeq highthroughput sequencing technology was used to sequence and analyze the 16S rRNA sequence of bacteria in the rhizosphere soil of 1-year-old(1Y),2-year-old(2Y),3-year-old(3Y)and 4-year-old(4Y)C.tangshen.The results were as follows:(1)The growth years significantly affected the bacterial community diversity in the rhizosphere soil of C.tangshen.The OTU number and alpha diversity index were increased first and then decreased with the increase of growth years;(2)Growth years changed the composition of bacterial community.However,at the phylum level,the dominant bacteria(relative abundance>5%)were relatively stable,including Proteobacteria,unclassified_Bacteria,Acidobacteria,Actinobacteria,Bacteroidetes and Chloroflexi,with a total relative abundance of 85.71%-91.39%.At the genus level,the dominant bacteria differed greatly.Only Holophaga and Candidatus Udaeobacter were the dominant genera coexisted in the four years.Gramnegative bacterium cTPY-13 was a unique dominant genus of 2Y,and the rest had corresponding dominant genera in different years;(3)The PCoA analysis and UPGMA cluster analysis showed that there were significant differences in bacterial communities among different growth years.With the increase of growth years,the differences in bacterial community structure among different groups was increased first and then decreased gradually,and the bacterial community evolved violently at 2-3 years old;(4)Soil available nutrients were the main environmental factors affecting the rhizosphere soil bacterial community,and the degree was hydrolyzable nitrogen(HN)>available phosphorus(AP)>available potassium(AK).Among the dominant bacteria,Acidobacteria was significantly negatively correlated with AP and AK(P<0.05),Bacteroidetes was significantly positively correlated with AP and AK(P<0.05),while Chloroflexi was significantly negatively correlated with HN(P<0.05).All the above results indicate that the extension of growth years of C.tangshen will affect the bacterial community structure and diversity of rhizosphere soil,thus changing its rhizosphere microecosystem.
作者
罗敏
朱晓富
徐广
罗舜
卢胜鄂
郭瑀
杨丹
邓才富
LUO Min;ZHU Xiao-fu;XU Guang;LUO Shun;LU Sheng-e;GUO Yu;YANG Dan;DENG Cai-fu(Chongqing Institute of Medicinal Plant Cultivation,Chongqing 408435;Chongqing College of Traditional Chinese Medicine,Chongqing 402760)
出处
《中国土壤与肥料》
CAS
CSCD
北大核心
2024年第3期32-39,共8页
Soil and Fertilizer Sciences in China
基金
重庆市自然科学基金面上项目(cstc2020jcyj-msxmX0470)
重庆市科研机构绩效激励引导专项(cstc2021-jxjl007,cstc2018jxjl-jbky130019)
重庆市科技局基本科研业务费专项项目(23JK78-2312)。
关键词
川党参
根际土壤
细菌群落
高通量测序
Codonopsis tangshen Oliv.
rhizosphere soil
bacterial community
high-throughput sequencing
