摘要
采用相分离法分别制备了含蛋白酶和不含蛋白酶的两种丝素蛋白微球,并将这两种微球按不同配比包裹至以钌化物光交联的丝素蛋白水凝胶内部,研究该凝胶降解速率对其表面黏附细胞增殖的影响。通过质量损失法表征水凝胶的降解速率,用扫描电镜和电子万能试验机表征降解速率对水凝胶微观结构和压缩力学性能的影响,通过CCK-8法评估水凝胶降解速率对其表面细胞增殖的影响。研究结果表明:丝素蛋白水凝胶的降解使其表面形貌变得粗糙起伏、并逐渐变硬、变脆,当水凝胶内部包裹的不含酶微球和含酶微球质量比为7∶3时,其表面培养的细胞增殖最快。
At present,there is a lack of research on the effect of silk fibroin(SF)hydrogel degradation rate on the behavior of cells.To solve the problem,SF microspheres with or without protease were prepared through phase separation method,and were encapsulated into SF hydrogels photocrosslinked by ruthenium compounds.Through the proportion regulation of the microspheres,the effective control of SF hydrogel degradation rates could be achieved.Based on the construction of SF hydrogels with different degradation rates,NIH3T3 cells were selected to investigate the effect of SF hydrogel degradation rate on the proliferation of cells adhering on its surface.The hydrogel degradation rates were verified by the corresponding mass loss during the incubation period.Further researches were conducted on the effects of SF hydrogel degradation rates on the microstructure and compressive mechanical properties by using scanning electron microscopy and electronic universal testing machine.In addition,the effect of different hydrogel degradation rates on the proliferation of cells cultured on its surface was evaluated by CCK-8 method.Results showed that the degradation rate of SF hydrogel could be feasibly and effectively regulated.The degradation rate was proportional to the ratio of encapsulated microspheres with protease.After 6 d of incubation,the fastest degradation hydrogel lost about 40%of its original mass.The microscopic network structure of hydrogel was severely damaged,and the surface morphology changed significantly from smooth to rough.The mechanical properties of the hydrogel gradually became hard and brittle from the initial soft elastomer.On the contrary,the weight loss of the slowest degradation hydrogel group was only about 10%,and the changes of microstructure and mechanical properties were relatively slight.Cell experiments showed that the fastest proliferation of cells occurred on the SF hydrogel with moderate degradation rate at the encapsulation ratio of the microspheres without protease to the microspheres with protease of 7∶3.This study provides a valuable reference for the controlling and constructing of tissue engineering scaffolds with appropriate degradation rates.
作者
祝天浩
蔡国龙
黄亮
胥学谦
姚响
张耀鹏
ZHU Tianhao;CAI Guolong;HUANG Liang;XU Xueqian;YAO Xiang;ZHANG Yaopeng(State Key Laboratory for Modification of Chemical Fibers and Polymer Materials,College of Materials Science and Engineering,Donghua University,Shanghai 201620,China)
出处
《功能高分子学报》
CAS
CSCD
北大核心
2024年第3期215-222,共8页
Journal of Functional Polymers
基金
中央高校基本科研业务费专项资金(2232024D-01)
上海市科学技术委员会资助项目(22520711900)
国家自然科学基金(52273125,52173031)。
关键词
丝素蛋白水凝胶
丝素蛋白微球
蛋白酶
材料降解调控
细胞增殖
silk fibroin hydrogel
silk fibroin microsphere
protease
material degradation regulation
cell proliferation
