摘要
[目的]本文旨在探究硝化作用强度对强化生物除磷(EBPR)工艺运行效果的影响。[方法]采用厌氧-好氧型序批式生物反应器(SBR)驯化得到EBPR污泥,将进水氨氮浓度提高至48 mg·L^(-1),比较无硝化抑制剂和添加硝化抑制剂2种条件下EBPR系统中硝化作用强度及除磷效率的变化,分析硝化作用强度对EBPR工艺运行效果的影响。在系统运行各阶段均取1个周期,测定混合液中磷酸根、氨氮、硝氮、亚硝氮质量浓度以及EBPR污泥中糖原、聚羟基烷酸(PHA)含量的周期变化,分析硝化作用强度对聚磷菌代谢的影响。[结果]在进水氨氮浓度提高至48 mg·L^(-1)且无硝化抑制剂的情况下,硝化作用强度逐渐增强,17 d后硝化反应速率上升至1.45~1.80 mg·h^(-1)·g^(-1),系统出现了好氧磷释放现象,导致除磷效率大幅下降,同时出现糖原的降解量与合成量显著上升、PHA的合成量与降解量显著下降等代谢异常现象;再次加入硝化抑制剂,系统的除磷功能在24 h内恢复正常。[结论]EBPR工艺运行效果与硝化作用强度密切相关,当硝化反应速率上升到一定程度时可引起好氧磷释放现象,导致EBPR工艺除磷效率大幅下降。
[Objectives]The present study aimed to investigate the effect of nitrification intensity on enhanced biological phosphorus removal(EBPR)process performance.[Methods]Acclimation of EBPR sludge was firstly carried out in an anaerobic-aerobic sequencing batch reactor(SBR).The influent ammonia concentration was raised to 48 mg·L^(-1)and a comparative study was conducted on the nitrification intensity and phosphorus removal efficiency of EBPR system with or without nitrification inhibitors addition for the purpose of further analyzing the effect of nitrification intensity on the operational performance of EBPR process.In each of those operational phases,cyclic changes of phosphate,ammonia,nitrate,nitrite concentrations in the mixed liquor,glycogen and polyhydroxyalkanoate(PHA)contents in EBPR sludge were determined to analyze the effect of nitrification intensity on the metabolism of polyphosphate accumulating organisms(PAOs).[Results]When the influent ammonia concentration raised to 48 mg·L^(-1)and with allylthiourea eliminated,the nitrification rate of EBPR system rose to 1.45-1.80 mg·h^(-1)·g^(-1)after 17 days of operation,and an unexpected phenomenon of aerobic phosphorus release occurred,leading to a substantial decrease in the phosphorus removal efficiency of the system.Metabolic abnormality also occurred in PAOs as indicated by a significant increase in the amount of glycogen degradation and synthesis and a significant decrease in the amount of PHA synthesis and degradation.However,EBPR performance was restored to the normal level within 24 h following re-supplementation of allylthiourea to the influent.[Conclusions]EBPR process performance was closely related to the nitrification intensity and aerobic phosphorus release occurred as nitrification rate rose up to a certain degree,leading to a significant decline in phosphorus removal efficiency of EBPR process.
作者
王可馨
王世明
顾向阳
WANG Kexin;WANG Shiming;GU Xiangyang(College of Life Sciences/Key Lab of Microbiological Engineering for Agricultural Environments,Ministry of Agriculture and Rural Affairs,Nanjing Agricultural University,Nanjing 210095,China;School of Environmental&Biological Engineering,Nanjing University of Science&Technology,Nanjing 210094,China)
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2024年第3期462-467,共6页
Journal of Nanjing Agricultural University
基金
国家自然科学基金项目(31970121)。
关键词
强化生物除磷
糖原
聚羟基烷酸
硝化反应速率
好氧磷释放
enhanced biological phosphorus removal
glycogen
polyhydroxyalkanoate
nitrification rate
aerobic phosphorus release
