摘要
为了筛选获得香蕉根际土壤中的高效固氮菌,本研究利用Ashby无氮培养基从海南省霸王岭野生香蕉(Musa nana Lour.sp)根际土壤中分离了一株固氮菌,并命名为BWLY3X-6,对其进行形态特征、生理生化、16S r RNA和gyr B序列分析、固氮特性及对不同香蕉品种的促生能力研究。结果表明:经鉴定,菌株BWLY3X-6属于固氮菌Klebsiella variicola,其胞外分泌氨态氮含量为(17.98±1.88)μg/m L,固氮酶活性为(220.51±8.21)ng/L;菌株对不同香蕉品种的促生长试验结果表明,与空白对照和0.5%尿素处理相比,在BWLY3X-6菌体10^(6)CFU/mL与10^(8)CFU/mL浓度下,南天黄与巴西蕉的各项生物量均有显著变化(P<0.05),其中根长变化量更加明显,分别增加43.5%和54.6%。本研究筛选获得了具有良好固氮能力的固氮菌,且该菌株对香蕉具有良好的促生作用,为其后续在农业生产中的实际应用提供理论基础。
A nitrogen fixing bacteria strain named BWLY3X-6 was isolated from the rhizosphere soil of wild banana(Musa nana Lour.sp)in Hainan,China using Ashby nitrogen free medium,and.its morphological characteristics,physiological and biochemical characteristics,16S rRNA and gyrB sequence analysis,nitrogen fixation characteristics and growth promoting ability to different banana varieties were studied.The strain was identified as an azotobacter Klebsiella variicola,and its extracellular ammonia nitrogen content was(17.98±1.88)μg/mL,nitrogenase activity was(220.51±8.21)ng/L.The results of the growth promoting test of the strain on different banana varieties showed that,compared with the blank control and 0.5%urea treatment,after inoculation with BWLY3X-6,under the inoculation concentration of 10^(6) CFU/mL and 10^(8) CFU/mL,there were significant changes in various biomass of M.acuminata AAA Cavendish cv.Nantianhuang and M.acuminata AAA Cavendish cv.Baxi(P<0.05),with more significant changes in root length,with the increase ratio of 43.5%and 54.6%respectively.In this study,nitrogen fixing bacteria with good nitrogen fixing ability were screened,and this strain had a good promoting effect on banana growth,wihich would pro-vide a theoretical basis for its subsequent practical application in agricultural production.
作者
徐云龙
周游
汪军
郭立佳
黄俊生
杨腊英
XU Yunong;ZHOU You;WANG Jun;GUO Lijia;HUANG Junsheng;YANG Laying(School of Plant Protection,Hainan University,Haikou,Hainan 570228,China;Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Integrated Pest Management of Tropical Crops,Ministry of Agriculture and Rural Affairs/Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests,Haikou,Hainan 571101,China)
出处
《热带作物学报》
CSCD
北大核心
2024年第5期936-943,共8页
Chinese Journal of Tropical Crops
基金
国家重点研发计划项目(No.2021YFC2600400)
中央级公益性科研院所基本科研业务费专项(No.1630042022010)
中国热带农业科学院国家热带农业科学中心科技创新团队项目(No.CATASCXTD202312)。
