摘要
目的分析血清正五聚体蛋白-3(PTX-3)联合沉默信息调节因子2相关酶1(SIRT1)对缺血性肠病(IBD)的诊断及预后价值。方法选取2020年12月至2022年12月四川省成都市新都区第三人民医院消化内科收治的126例IBD患者作为观察组,另选取同期在四川省成都市新都区第三人民医院进行体检的98例健康者作为对照组。对观察组患者进行为期一年的随访调查,根据患者预后生存情况分为预后良好组(69例)和预后不良组(57例)。采用酶联免疫吸附试验检测所有研究对象的血清PTX-3、SIRT1水平。比较观察组和对照组的血清PTX-3、SIRT1水平,以及预后良好组和预后不良组的临床资料。采用多因素Logistic回归分析IBD患者预后不良的危险因素;采用Pearson相关分析IBD患者血清PTX-3水平与SIRT1水平的相关性;绘制受试者工作特征(ROC)曲线分析血清PTX-3、SIRT1单独及2项指标联合检测对IBD的诊断价值。结果观察组血清PTX-3水平高于对照组,血清SIRT1水平低于对照组,差异均有统计学意义(P<0.05)。预后不良组的体质量指数、PTX-3水平均高于预后良好组,SIRT1水平低于预后良好组,差异均有统计学意义(P<0.05)。多因素Logistic回归分析结果显示,血清PTX-3水平上升、血清SIRT1水平下降是IBD患者预后不良的危险因素(P<0.05)。Pearson相关分析结果显示,IBD患者血清PTX-3水平与SIRT1水平呈负相关(r=-0.447,P<0.001)。ROC曲线分析结果显示,2项指标联合检测诊断IBD的曲线下面积(AUC)大于血清PTX-3、SIRT1单独诊断IBD的AUC(Z=2.827、2.712,P=0.004、0.007)。结论IBD患者血清PTX-3水平升高、SIRT1水平降低,2项指标水平与IBD患者的预后密切相关,且2项指标联合检测对IBD具有较高的诊断价值。
Objective To analyse the diagnostic and prognostic value of serum pentraxin-3(PTX-3)combined with silencing information regulator 2 related enzyme 1(SIRT1)in ischemic bowel disease(IBD).Methods A total of 126 patients with IBD admitted to the Gastroenterology Department of Chengdu Municipal Xindu District Third People′s Hospital from December 2020 to December 2022 were selected as the observation group,and 98 healthy people who underwent physical examination in Chengdu Municipal Xindu District Third People′s Hospital during the same period were selected as the control group.The observation group was followed up for 1 year and divided into good prognosis group(69 cases)and poor prognosis group(57 cases)according to the survival of the patients.Serum PTX-3 and SIRT1 levels of all subjects were detected by enzyme-linked immunosorbent assay.The serum PTX-3 and SIRT1 levels of the observation group and the control group were compared,as well as the clinical data of the good prognosis group and the poor prognosis group.Multivariate Logistic regression was used to analyze the risk factors of poor prognosis in IBD patients.Pearson correlation was used to analyze the correlation between serum PTX-3 level and SIRT1 level in IBD patients.Receiver operating characteristic(ROC)curve was drawn to analyze the diagnostic value of serum PTX-3 and SIRT1 alone and combined with the two indexes in IBD.Results The serum PTX-3 level in the observation group was higher than that in the control group,and the serum SIRT1 level in the observation group was lower than that in the control group,and the differences were statistically significant(P<0.05).The body mass index and PTX-3 level in the poor prognosis group were higher than those in the good prognosis group,and the SIRT1 level in the poor prognosis group was lower than that in the good prognosis group,and the differences were statistically significant(P<0.05).Multivariate Logistic regression analysis showed that the increase of serum PTX-3 level and the decrease of serum SIRT1 level were risk factors for poor prognosis of IBD patients(P<0.05).Pearson correlation analysis showed that serum PTX-3 level was negatively correlated with SIRT1 level in IBD patients(r=-0.447,P<0.001).ROC curve analysis results showed that the area under the curve(AUC)of the combined with the two indexes diagnosis of IBD was greater than that of serum PTX-3 and SIRT1 alone in diagnosis of IBD(Z=2.827,2.712,P=0.004,0.007).Conclusion Serum PTX-3 level increased and SIRT1 level decreased in patients with IBD,the two indexes levels are closely related to the prognosis of patients with IBD,and the combined detection of the two indexes has high diagnostic value in the IBD.
作者
章丽
朱利华
蒲杉杉
ZHANG Li;ZHU Lihua;PU Shanshan(Department of Gastroenterology,Chengdu Municipal Xindu District Third People′s Hospital,Chengdu,Sichuan 610504,China;Department of Gastroenterology,Panzhihua Municipal Renhe District People′s Hospital,Panzhihua,Sichuan 617005,China)
出处
《检验医学与临床》
CAS
2024年第11期1627-1630,1635,共5页
Laboratory Medicine and Clinic
