摘要
利用UHPLC-Q-Orbitrap四极杆-静电场轨道阱高分辨质谱联用技术对半夏的化学成分进行快速识别和鉴定。以80%(V/V)甲醇提取,Acquity UPLC BEH C18色谱柱(2.1 mm×100 mm,1.7μm)分离,0.1%甲酸水(A)-0.1%甲酸的乙腈(B)溶液作为流动相进行梯度洗脱。质谱采用正、负离子监测模式,利用全扫描及自动触发二级质谱扫描,快速识别和鉴定半夏中的化学成分。根据高分辨质谱数据,从半夏中共分析鉴定出111个化合物,包括生物碱类37个、氨基酸及其衍生物20个、黄酮类17个、萜类12个、有机酸及其衍生物6个、苯丙素类5个、其它类14个。该方法可为半夏质量控制和药效物质研究提供参考。
An UHPLC-Quadrupole-Orbitrap high resolution mass spectrometry method was developed to rapidly identify the chemical components from Pinelliae Rhizoma by using 80%(V/V)methanol as the extraction solution.For UHPLC separation,Acquity UPLC BEH C18 column(2.1 mm×100 mm,1.7μm)was used,and gradient elution was performed with 0.1%formic acid water(A)-0.1%formic acid acetonitrile(B)solution as mobile phase.The chemical components in Pinelliae Rhizoma were quickly identified by the mass spectrometry adopting positive and negative ion monitoring modes,full scanning and automatic triggering of secondary mass spectrometry.As a result,a total of 111 compounds have been identified from Pinelliae Rhizoma,including 37 kinds of alkaloids,20 kinds of amino acids and derivants,17 kinds of flavonoids,12 kinds of terpenoids,6 kinds of organic acids and derivants,5 kinds of phenylpropanoids,and 14 other kinds of compounds.The method provides a reference for quality control and pharmacodynamic material basics.
作者
王静
崔洁
刘子怡
杨杨
李展
刘慧灵
WANG Jing;CUI Jie;LIU Ziyi;YANG Yang;LI Zhan;LIU Huiling(Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Beijing 100193,China)
出处
《分析试验室》
EI
CAS
CSCD
北大核心
2024年第5期711-718,共8页
Chinese Journal of Analysis Laboratory
