摘要
研究唐古特白刺叶对3%葡聚糖硫酸钠(DSS)诱导的溃疡性结肠炎小鼠的缓解作用及可能的作用机制.采用DSS诱导小鼠溃疡性结肠炎,用唐古特白刺叶提取物给予治疗,通过HE染色法、疾病活动指数(DAI)、结肠组织病理学评分、酶联免疫吸附测定法(ELISA)检测血清中炎性因子IL-6、IL-1β、TNF-α的水平,并采用HPLC-ESI-HRMS/MS对白刺叶提取物进行化学成分指认和鉴定,在化学成分的基础上,基于HepG-2细胞对活性氧(ROS)、谷胱甘肽转移酶(GSH)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)等抗氧化酶水平进行检测.与正常组比较,模型组结肠显著缩短,DAI评分显著增加,血清中IL-6、IL-1β、TNF-α水平(P<0.01)升高,在HepG-2细胞中,模型组活性氧(ROS)水平升高,GSH、SOD、CAT降低(P<0.05);与模型组比较,白刺叶提取物组小鼠DAI评分和血清中IL-6、IL-1β、TNF-α水平降低(P<0.01),溃疡性结肠炎症状减轻,且在HepG-2细胞中,白刺叶提取物组ROS水平降低,GSH、SOD、CAT水平升高(P<0.05).唐古特白刺叶对DSS诱导的溃疡性结肠炎具有治疗作用,其作用机制可能与降低炎症因子和活性氧水平,同时升高抗氧化酶密切相关.
The study aims to explore the alleviating effect of Nitraria Tangutorum Bobr.leaves on mice with DSS in⁃duced ulcerative colitis and its possible mechanism.Mice with ulcerative colitis induced by DSS were treated with the extract of Nitraria Tangutorum Bobr.leaves,the histopathological changes of colon were observed by HE staining and disease activity index(DAI),and enzyme⁃linked immunosorbent assay(ELISA)was used to detect the content of inflammatory factors IL⁃6、IL⁃1β、TNF⁃α.In addition,HPLC⁃ESI⁃HRMS/MS was used to identify the chemical components of Nitraria Tangutorum Bobr.Leaves.On the basis of the chemical components,the levels of antioxidant enzymes such as reactive oxygen species(ROS),glutathione transferase(GSH),superoxide dismutase(SOD)and catalase(CAT)were detected based on HepG⁃2 cells.Compared with normal group,colon DAI score and serum levels of IL⁃6,IL⁃1βand TNF⁃αwere increased in the model group(P<0.01).ROS levels were increased in the model group,while GSH,SOD and CAT were decreased in the HepG⁃2 cells(P<0.05).Compared with the model group,DAI,IL⁃6,IL⁃1βand TNF⁃αlevels in serum were decreased(P<0.01),symptoms of ulcerative colitis were alleviated,ROS levels in HepG⁃2 cells were decreased,and GSH,SOD and CAT levels were increased in Nitraria Tangutorum Bobr.leaves group(P<0.05).Conclusion Nitraria Tangutorum Bobr.leaves have therapeutic effects on DSS induced ulcerative colitis,and the mechanism of action may be closely related to reducing the levels of in⁃flammatory factors and active oxygen,increasing the levels of antioxidant enzymes at the same time.
作者
濮兴娜
冯健
郭晶
刘明秀
王煜丹
李艳平
PU Xing-na;FENG Jian;GUO Jing;LIU Ming-xiu;WANG Yu-dan;LI Yan-ping(School of Traditional Chinese Medicine,Yunnan University of Chinese Medicine,Kunming 650500,China;National and Local Joint Engineering Research Center for Green Preparation Technology of Biobased Materials,Yunnan Minzu University,Kunming 650500,China)
出处
《云南民族大学学报（自然科学版）》
CAS
2024年第3期275-285,共11页
Journal of Yunnan Minzu University:Natural Sciences Edition
基金
云南省应用基础研究计划面上项目(202201AT070228)
云南省应用基础研究计划中医联合专项重点项目(202101AZ070001-326).
