摘要
通过苦瓜枯萎病抗性生理研究,构建高效精准的苦瓜枯萎病抗性鉴定评价体系,旨在为苦瓜抗枯萎病种质资源筛选及抗病品种选育提供理论基础。以热科2号苦瓜为试验材料,选用灌根法、胚根法和伤根法在苦瓜2叶1心期接种不同浓度的苦瓜枯萎病菌,分析不同接种时间的病情指数和生理响应的差异情况,以及不同接种方法和接种浓度对病害的影响。结果表明,采用灌根法接种1×10^(6)个·mL^(-1)的枯萎病菌,在第25天时病情指数为37.1,显著低于1×10^(7)个·mL^(-1)、显著高于1×10^(4)个·mL^(-1),苯丙氨酸解氨酶活性和叶绿素含量显著低于胚根法,多酚氧化酶活性与可溶性蛋白含量与胚根法相比无显著差异。在苦瓜幼苗2叶1心时采用灌根法接种1×10^(6)个·mL^(-1)的枯萎病菌,25 d时鉴定发病情况,可作为精准高效鉴定苦瓜枯萎病抗性的体系。
Through the study of bitter gourd Fusarium wilt resistance physiology,the authors have constructed an efficient and precise identification and evaluation system for bitter gourd Fusarium wilt resistance,aiming to provide a theoretical basis for the screening and evaluation of Fusarium-resistant germplasm resources and the selection and breeding of disease-resistant varieties.In this study,Reke No.2 bitter gourd was used as the test material,and selected the root irrigation,radicle root and injure root methods to inoculate different concentrations of Fusarium wilt fungus liquid at the time of two-leaf and one heart of bitter gourd,and investigated the effects of different inoculation methods and inoculation concentrations on the Fusarium wilt by analyzing the differences in disease index and physiological responses at different inoculation times.The results showed that the disease index was 37.1 using the root irrigation inoculated fungus with 1×10^(6)·mL^(-1) at 25 d,which was significantly lower than that of 1×10^(7)·mL^(-1),and significantly higher than that of 1×10^(4)·mL^(-1).PAL activity and chlorophyll content were significantly lower than the radicle method,and no significant difference existed between PPO activity and soluble protein content.When bitter gourd seedlings of two-leaf and one heart were inoculated with 1×10^(6)·mL^(-1) fungus using the root irrigation method,the disease incidence was identified at 25 d,which can serve as an accurate and efficient identification system of bitter gourd Fusarium wilt resistance.
作者
凌怡
杨衍
牛玉
于仁波
刘昭华
刘平武
韩旭
LING Yi;YANG Yan;NIU Yu;YU Renbo;LIU Zhaohua;LIU Pingwu;HAN Xu(College of Tropical Agroforestry,Hainan University,Haikou 570228,Hainan,China;Tropical Crop Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,Hainan,China)
出处
《中国瓜菜》
CAS
北大核心
2024年第6期69-78,共10页
China Cucurbits And Vegetables
基金
海南省重点研发项目(ZDYF2024XDNY238)
海南省重大科技计划项目(ZDKJ2021010)
海南省冬季瓜菜产业技术体系首席科学家项目(HNARS-05-SX)
海南省冬季瓜菜产业技术体系遗传育种岗岗位专家项目(HNARS-05-G02)
海南省高层次人才项目(320RC7230)。
关键词
苦瓜
枯萎病
病情指数
抗性生理
鉴定评价技术
Bitter gourd
Fusarium wilt
Disease index
Resistance physiology
Identification and evaluation technology
