摘要
以东魁幼苗为试材,研究3种诱导剂(枯草芽孢杆菌、水杨酸、芸苔赤霉酸)对杨梅凋萎病抗性及生长发育的影响。结果表明,在枯草芽孢杆菌和芸苔赤霉酸处理下,杨梅凋萎病病情指数显著低于对照组,相对诱导效果分别为35.44%、27.16%;在枯草芽孢杆菌、芸苔赤霉酸诱导处理下,叶长、叶面积均高于对照,叶面积较对照分别提高8.82%、8.42%;3种诱导剂处理下,叶绿素含量均有不同程度增加,在枯草芽孢杆菌处理下叶绿素a、叶绿素b含量均显著高于对照;超氧化物歧化酶、过氧化物酶、过氧化氢酶等抗氧化酶活性在3种诱导剂处理下,均有显著提高,其中枯草芽孢杆菌、芸苔赤霉酸的作用较为显著,且其对应丙二醛含量显著低于对照。综上,研究筛选到能诱导杨梅凋萎病抗性的2种诱导剂,即枯草芽孢杆菌和芸苔赤霉酸,可为今后杨梅凋萎病的防控及相关研究提供重要依据。
In this study,using Dongkui seedlings as test materials,the effects of Bacillus subtilis(BS),salicylic acid(SA),and brassinolactone·indoleacetic acid·gibberellic acid(BHG)induced treatment on the resistance of twig blight disease and growth of Chinese bayberry were investigated.The results showed that when compared with the control group,the disease index was significantly lower under BS and BHG treatment,and their relative induction effects were 35.44%and 27.16%,respectively.Under the induction of BS and BHG,both leaf length and leaf area were higher than the control,with the leaf area increased by 8.82%and 8.42%,respectively.Under the treatment of all three inducers,the chlorophyll content increased to varying degrees.Under the treatment of BS,the content of chlorophyll a and chlorophyll b were significantly higher than the control.The activities of antioxidant enzymes including SOD,POD and CAT were significantly increased under the treatment of three inducers.Among them,the induction effects of BS and BHG were more significant,and their corresponding MDA content was significantly lower than the control.In summary,this study identified two inducers including BS and BHG that can induce resistance to twig blight disease,which can provide important basis for the prevention and control of twig blight disease in Chinese bayberry in the future.
作者
丁娟
孙学鹏
俞浙萍
张淑文
孙鹂
梁森苗
戚行江
DING Juan;SUN Xuepeng;YU Zheping;ZHANG Shuwen;SUN Li;LIANG Senmiao;QI Xingjiang(College of Horticulture Science,Zhejiang A&F University,Hangzhou 311300,Zhejiang;Institute of Horticulture,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,Zhejiang;Xianghu Lab,Hangzhou 311231,Zhejiang)
出处
《浙江农业科学》
2024年第6期1424-1428,共5页
Journal of Zhejiang Agricultural Sciences
基金
浙江省重点研发计划(2021C02009)
上虞地方科技合作项目(SY202305)
黄岩农业“揭榜挂帅”项目(JBGS2022-HYNY01)。
关键词
杨梅
凋萎病
诱导剂
Chinese bayberry
twig blight disease
inducers
